小黑豆乳清蛋白和水解多肽对小鼠急性肝损伤的保护作用

Protective Effect of Whey Protein and Polypeptide from Small Black Soybean on Acute Liver Injury in Mice

目的:研究小黑豆乳清蛋白(SBWP)和水解多肽(SBWPE)对四氯化碳(CCl4)引起的小鼠肝脏损伤的保护作用。方法:分别将SBWP和SBWPE按照6g/(kg.d)和2g/(kg.d)加入到饲料中,饲喂小鼠30d。实验第30天,CCl4经胃给药(5mL/kg)建成急性肝损伤模型;实验结束后,处死动物,检测血清谷草转氨酶(AST)、谷丙转氨酶(AST)和肝脏丙二醛(MDA)、甘油三酯(TG)、谷胱甘肽(GSH)。结果:与模型组相比,SBWP和SBWPE组动物的血清ALT活性和肝脏MDA、TG含量显著降低(P<0.01),肝中GSH含量显著升高(P<0.05),其中以SBWPE组效果最明显。结论:SBWP和SBWPE对CCl4引起的小鼠急性肝损伤有保护作用。

Objective：To explore the protective effect of whey protein and polypeptide from small black soybean on CCl4-induced acute hepatic injury in mice.Methods：The protein and polypeptide were added to mice feed at the dose of 6 g/（kg？d） and 2 g/（kg？d）,respectively,which were fed to the mice for 30 d.Mouse model of acute hepatic injury was established by intragastric feeding with 1% CCl4（80 mg/kg）.The mice were sacrificed at the end of the experiment.The effect of the protein and polypeptide on serum AST and ALT activities and liver GSH,TG and MDA contents was observed.Results：Compared with the model group,whey protein and polypeptide could decrease ALT activity in mice in serum and TG and MDA levels in mouse liver significantly（P＆lt;0.01）,and increase GSH content in mouse liver（P＆lt;0.01）.The polypeptide at high dosage revealed the most obvious effect.Conclusion：Whey protein and polypeptide from small black soybean have protective capability against acute liver injury in mice induced by CCl4.