中华真地鳖酶解短肽的抗氧化作用

Antioxidant Activity of Eupolyphaga sinensis Peptides

目的:研究中华真地鳖酶解短肽的体内外抗氧化作用。方法:测定中华真地鳖酶解短肽体外对.OH、O2-.和DPPH自由基的清除作用;并将中华真地鳖酶解短肽灌胃,颈背部皮下注射D-半乳糖致衰老模型小鼠,以小鼠血浆和肝脏中丙二醛(MDA)含量、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(T-SOD)及清除.OH活性为指标,研究其体内抗氧化活性。结果:中华真地鳖酶解短肽对.OH、O2-.和DPPH自由基有较强的清除作用,其IC50分别为0.40、8.73、1.32mg/mL;并且与模型组相比,中华真地鳖酶解短肽能显著降低模型小鼠血浆和肝脏中MDA含量(P<0.01),显著提高其CAT活力(P<0.05)、GSH-Px活力(P<0.01)及清除.OH(P<0.05)能力,显著提高小鼠肝脏T-SOD活力(P<0.05)。结论:中华真地鳖酶解短肽有较强的体内外抗氧化作用。

Objective：To investigate the antioxidant activity of Eupolyphaga sinensis peptides in vivo and in vitro.Methods：The scavenging capacity of Eupolyphaga sinensis peptides against hydroxyl,superoxide anion,and DPPH free radicals in vitro were evaluated.The activities of CAT,GSH-Px and T-SOD,hydroxyl radical scavenging rate and the content of MDA in plasma and liver in D-galactose induced aging model mice were analyzed after feeding hydrolyzed peptides from Eupolyphaga sinensis.Results：Eupolyphaga sinensis peptides had strong scavenging effect on hydroxyl,superoxide anion and DPPH free radicals with IC50 of 0.40,8.73 mg/mL and 1.32 mg/mL,respectively.Compared to the aging model group,the peptides significantly reduced the content of MDA（P＆lt;0.01）,increased the activities of CAT and GSH-Px（P＆lt;0.05,P＆lt;0.01） and hydroxyl radical scavenging rate（P＆lt;0.05） in both plasma and liver,and also enhanced the activity of T-SOD in liver（P＆lt;0.05）.Conclusion：Eupolyphaga sinensis peptides have obvious antioxidant activity.