重组腺病毒Ad-Runx3对前列腺癌细胞增殖的影响
摘要
目的:探讨重组腺病毒介导的转录因子Runx3对PC-3前列腺癌细胞增殖的影响。方法:以Ad-Runx3为实验组,Ad-EGFP为对照组,应用MTT法、平板克隆形成实验观察Runx3对PC-3细胞的生长抑制作用;流式细胞术检测Runx3对PC-3细胞周期的阻滞作用。结果:腺病毒介导Runx3显著抑制PC-3前列腺癌细胞的体外生长能力及平板克隆形成能力,感染PC-3细胞36h后可检测到显著的细胞周期阻滞。结论:重组腺病毒介导的转录因子Runx3对PC-3前列腺癌细胞有显著的抑制生长和克隆形成、促进周期阻滞的作用,为前列腺癌的治疗提供了新策略。
出处
《实用医学杂志》
CAS
北大核心
2013年第5期700-702,共3页
The Journal of Practical Medicine
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