六元环蛋氨酸螯合硒的合成、表征及光谱特性

Synthesis and Spectral Characteristics of Selenium-Chelated Methionine with Hexagon

以蛋氨酸和无水乙醇为原料,对甲苯磺酸为催化剂,苯为携水剂先合成蛋氨酸酯化物;将蛋氨酸酯化物再与亚硒酸钠按一定比例反应,经低温陈化、结晶,可得到螯合硒的蛋氨酸。通过微电泳法测定产物的Zeta电位,计算出等电点并利用等电点法对产物分离提纯。利用红外光谱、紫外光谱、X射线衍射法、1H核磁共振法对产物光谱性质进行分析,确定所制备的蛋氨酸螯合硒是以蛋氨酸中的硫原子和氨基中的氮原子为配体,Se4+为中心离子形成了六元环蛋氨酸螯合硒。

Using methionine and anhydrous ethanol as raw material,p-toluene sulfonic acid as catalyst,and benzene as a carrying water agent,methionine ester was first synthesized.Then,selenium-chelated methionine was prepared through the reaction of methionine ester with sodium selenite by a certain proportion,settling and crystallizing at lower temperature.The ξ potential of the resultant was determined by micro-electrophoresis,through which the isoelectric point was calculated.Based on the principle of isoelectric point,it was separated and purified.The spectral properties of the resultant were analyzed by infrared spectrum,utraviolet spectrum,X ray diffraction analysis and 1H-NMR,from which we got the information of the resultant structure that has a hexagon composing of sulfur atom of methionine,nitrogen of amine and central selenium ion of four-valence.