摘要
目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导人非小细胞肺癌H596细胞发生凋亡,初步阐明非小细胞肺癌细胞经TRAIL诱导凋亡的作用和机制。方法:培养H596细胞,设对照组和不同浓度(0.01、0.03、0.10、0.30、1.00、3.00、10.00、30.00和100.00μg.L-1)TRAIL组,TRAIL作用H596细胞24h后,酸性磷酸酶法检测细胞凋亡的百分率;Western blotting法检测TRAIL相关蛋白caspase-8、Bcl-2及Fas相关死亡结构域(FADD)的表达情况。结果:TRAIL浓度为0.01~0.03μg.L-1时,对H596细胞的生长无明显抑制作用,细胞出现增殖趋势;从0.10μg.L-1开始,随着TRAIL浓度升高,细胞开始出现凋亡,至10.00μg.L-1时细胞凋亡水平显著增加,与对照组比较差异有统计学意义(P<0.05)。Western blotting检测,caspase-8、Bcl-2和FADD蛋白表达正常。结论:高浓度的TRAIL可以诱导H596细胞发生凋亡,可能与TRAIL相关蛋白表达正常有关联。
Objective To study the apoptosis induced by tumor necrosis factor related apoptosis-inducing ligand (TRAIL) in non-small cell lung carcinoma H596 cells, and to explore the role and mechanism of apoptosis. Methods Non-small cell lung carcinoma H596 cells were cultivated, and acid phosphate assay was used to detect the apoptotic rate, and the expressions of caspase-8, Bcl-2 and Fas-associated death domain (FADD) proteins were detected by Western blotting in H596 cells. Results The apoptotic rates of H596 cells were decreased after the cells were treated with 0.01--0.03 μg L-1 TRAIL, and from 0.10 μg L-1 TRAIL, the apoptotic rates were increased; the differences in apoptotic rates of H596 cells after treated with 10.00--100.00 μg L-1 TRAIL were significant compared with control group (P〈0.05). The Western blotting results showed that the expressions of caspase-8, Bcl-2, FADD proteins were normal. Oonclusion High dose of TRAIL can induce apoptosis in H596 cells, and it may be related to the normal expressions of TRAIL related proteins.
出处
《吉林大学学报(医学版)》
CAS
CSCD
北大核心
2013年第1期16-18,共3页
Journal of Jilin University:Medicine Edition
基金
国家自然科学基金资助课题(81201671)
吉林省教育厅项目资助课题(2012330
2012491)
关键词
癌
非小细胞肺
肿瘤坏死因子相关凋亡诱导配体
细胞凋亡
carcinoma, non-small cell lung
tumor necrosis factor related apoptosis-inducing ligand
apoptosis