自身免疫法制作大鼠慢性非细菌性前列腺炎模型的周期

The period needed to establish a rat model of chronic nonbacterial prostatitis using the autoimmune method

目的探讨应用前列腺组织蛋白提纯液（PTHS）辅以弗氏完全佐剂（FCA）和百白破疫苗（PDT），成功建立慢性非细菌性前列腺炎（CNP）大鼠模型所需的时间周期。方法取4个月龄雄性SD大鼠10只，麻醉处死后在无菌条件下剖腹取前列腺组织，高速离心制作PTHS，用微量紫外分光光度计测定前列腺组织蛋白含量，再以0．1mol／LpH7．4的PBS缓冲液调节蛋白浓度至20mg／ml。再将PTHS与FCA等体积混合成混悬液。另取2个月龄sD大鼠48只，随机分为6组，每组8只。其中5组为实验组（1周组、2周组、4周组、6周组、8周组），每只大鼠皮内多点注射PTHS与FCA的混悬液1．0ml，同时腹腔注射PDT0．5ml，分别于1,2,4,6、8周处死后取前列腺组织观察大体形态和光镜病理特征。另1组为对照组，同法注射等量生理盐水，并于第8周处死后观察前列腺特征。结果对照组大鼠前列腺大体形态正常，光镜下未见炎症表现；1周组大鼠前列腺也无明显炎症表现；2周组大鼠前列腺组织轻度充血水肿，腺体周围可见散在的淋巴细胞浸润；4周组大鼠前列腺结构出现轻中度破坏，间质、腺体内和腺体周围均出现较多的淋巴细胞浸润；6周组大鼠前列腺结构破坏严重，间质、腺体内和腺体周围有弥漫的淋巴样组织增生和淋巴、单核细胞等慢性炎细胞浸润，提示建模成功；8周组大鼠炎症情况与6周组大鼠类似。结论应用同源大鼠的PTHS辅以双重免疫佐剂（FCA和PDT）经过6周的时间可以成功建立CNP大鼠模型。

Objectives To investigate the period required to establish a rat model of chronic nonbacterial prostatitis（CNP） by immunized with syngeneic prostate tissue homogenate supemate（PTHS） plus Freund＇s complete adjuvant（FCA） and pertussis diphtheria tetanus vaccine（PDT）. Methods The prostate tissues of 10 of 4 -month - old male SD fats were taken out under aseptic condition, and PTHS was made through high - speed centrifugation. The concentration of PTHS was determined with micro UV spectrophotometer and diluted into 20 mg/ml then. Another 48 male SD rats of 4 months old were randomly divided into 6 groups with 8 rats each. Five groups were CNP group, including 1 - week group ,2 - week group,4 - week group ,6 - week group and 8 - week group. Each rat of group CNP was immunized with 1.0 ml PTHS emulsified by isopyknic FCA intradermally in the multiple points, and simultaneously immunized with 0.5 ml PDT vaccine intraperitoneally. Each rat of control group was injected with equivalent normal saline in the same way. The rats were sacrificed at 1,2,4,6 and 8 weeks after immunization in CNP group,and 8 weeks in control group. Then, The prostatic tissues were harvested under aseptic condition and examined macro- pathologically and histologically for degree of inflammation. Results Macroscopic and histological features of pros- tate tissues of control group were normal. The prostatic manifestations of 1 - week group were nearly normal too. But in the 2 -week group, the prostate tissues were mild hyperemia with scattered lymphocytic infiltration around the glands. In the g -week group, the prostate tissues were moderate hyperemia,with much lymphocytic infiltration with- in or around the acini or ducts. In the 6 - week group, the prostate tissues were serious congestion and edema, adhesion with surrounding tissues, aneretic prostate capsule and so on. Histologically, the prostatic tissues were characterized by lymphoid tissue proliferation and chronic inflammatory cell infiltration in the stromal connective tissue around the acini or ducts,suggesting success of modeling. The inflammatory conditions of the 4 - week group rats were similar to the rats of the 6 - week group. Conclusions Six weeks were needed to establish a rat model of CNP by immu- nized with PTHS plus FCA and PDT.