摘要
为确定白簕果实总黄酮的含量,采用超声辅助法提取白簕果实中的黄酮类化合物,以芦丁为对照,采用分光光度法在510nm处测定白簕果实中的总黄酮。回归方程为:A=1.0471C-0.0012,相关系数r=0.9997,精密度实验RSD为1.653%,稳定性实验(40min)RSD分别为2.011%(芦丁)和1.967%(白簕),平均加标回收率为101%,RSD=1.961%(n=6)。采用分光光度法,操作简便,结果准确,灵敏度高,重复性好,准确测定白簕果实中总黄酮含量为76.93mg/g。
Determination of the content of total flavonoids in fruit of A. trifoliatus（L. ） Merr. was studied. The total flavonoids in fruit of A. trifoliatus （L.） Merr. were extracted by ultrasound assisted method,ad then determined at 510nm by spectrophotometry with rutin as standard control. The result showed that the regression equation of rutin was A=1. 0471C-0. 0012,r=0. 9997,and the RSD for precision experiment was 1. 653%,RSDs for the stability experiment were 2. 011% （rutin） and 1. 967% [A. trifoliatus （L.） Merr. ]. The average recovery was 101%,with RSD of 1. 961% （n = 6）. The content of total flavonoids was determined by spectrophotometry, which was easy to operate with accurate result, high sensitivity and good reproducibility, and was used to determine total flavonoids in fruit of A. trifoliatus（L. ） Merr. with the accurate result of 76.93mg/g.
出处
《光谱实验室》
CAS
2013年第2期609-612,共4页
Chinese Journal of Spectroscopy Laboratory
基金
凯里学院服务地方经济项目(ZX1034)
凯里学院学生课题(X1117)
贵州省教育厅项目(黔教科[20010078])
贵州省重点学科建设基金资助(黔教科[2011]208)
关键词
白簕
果实
总黄酮含量
分光光度法
A. trifoliatus （L.） Merr.
Fruit
Content of Total Flavonoids
Spectophotometry
