摘要
建立了测定丹参酮ⅡA含量的高效液相色谱(HPLC)方法。方法采用Eclipse Plus C_(18)柱(250mm×4.6mm,5μm)色谱柱,流动相为70%的乙腈水溶液,流速为1.0mL·min^(-1),检测波长268nm,柱温为室温,进样量为20μL。丹参酮ⅡA在1.65×10^(-4)—1.0g·L^(-1)范围内与峰面积呈现良好的线性关系(r=0.9984),检出限为5.7×10^(-5)g·L^(-1)。所建立的方法简单、灵敏度高、重现性好,可用于制剂中丹参酮ⅡA含量的测定。
The method for determination of content of tanshinone Ⅱ A was developed by reversed-phase performance liquid chromatography. A Eclipse Plus C18 column was used with 70% acetonitrile water solution as mobile phase at a flow rate of 1.0mL ·min^-1. The detection wavelength was set at 268 nm and column temperature was room temperature. The injection volume was 20 μL. The tanshinone lr A showed a good linearity in the range of 1, 65 × 10^-4-1.0 g · L^-1 with peak area, the correlation coefficients was 0. 9984 and the detection limit was 5. 7 × 10^-5g · L^-1. The method is rapid with high sensitivity and good repeatability, and suitable for the determination of content of tanshinone 11 A in preparation.
出处
《光谱实验室》
CAS
2013年第2期795-797,共3页
Chinese Journal of Spectroscopy Laboratory