依达拉奉对肺泡Ⅱ型上皮细胞凋亡的保护作用

The protective effects of edaravone on lung epithelial type Ⅱ cells against apoptosis

目的探讨依达拉奉(EDA)对氧化应激诱导的肺泡Ⅱ型上皮细胞凋亡的影响及可能机制。方法用过氧化氢氧化应激建立氧化应激肺泡Ⅱ型上皮细胞凋亡模型细胞。将细胞分为:过氧化氢处理组(H组)、依达拉奉-过氧化氢处理组(E组)和对照组(C组)。采用脂质过氧化物(MDA)、谷胱甘肽(GSH)试剂盒检测A549细胞MDA、GSH的含量,同时测定三组细胞生长的抑制率、凋亡率、半胱氨酸天冬氨酸蛋白酶(caspase)-3与caspase-9mRNA及蛋白表达。结果与C组比较,H组细胞MDA、细胞生长抑制率、凋亡指数、caspase-3与caspase-9mRNA相对表达及蛋白表达量明显升高;而GSH明显降低(P<0.01)。与H组比较,E组细胞MDA、细胞生长抑制率、凋亡指数、caspase-3与caspase-9mRNA相对表达及蛋白表达量明显降低;而GSH明显增高(P<0.05)。结论依达拉奉可通过抗氧化及抗caspase-3,caspase-9引起的凋亡以保护肺泡Ⅱ型上皮细胞对抗氧化应激。

Objective Our present study was undertaken to explore the effects of edaravone on lung epithelial type Ⅱ cells against apoptosis induced by oxidative stress. Methods Lung epithelial typeⅡ cells with H202 treatment were divided into three groups： hydrogen peroxide group （group Ⅱ）, edaravonetreated hydrogen peroxide group （group E） and control group （group C）. Malonic dialed hyde （MDA）, glutathione （GSH）, cell inhibition ration, apoptosis rate, caspase-3, and caspase-9 were determined in each group. Results Compared with group C, cell inhibition ration was significantly increased accompanied by elevated MDA content, apoptosis rate, caspase-3, and caspase-9 mRNA and protein expression in group H, while the content of GSH were reduced（P〈0. 01）. Compared with group H, the MDA, cell inhibition, almptosis rate, caspase-3 and caspase-9 mRNA and protein were decreased while GSH were increased in group E（P〈0. 05）. Conclusion Edaravone can reduce apoptosis of lung epithelial type Ⅱ ceils induced by oxidative stress, which might be due to the inhibition of caspase-3 and caspase-9 expressiorL