响应曲面法优化Lipozyme TL IM催化大豆油制备1,3-甘油二酯

Optimization of production condition of diacylglycerol from soybean oil hydrolyzed by Lipozyme TL IM by response surface methodology

为优化固定化脂肪酶LipozymeTLIM催化大豆油制备高纯度1,3-甘油二酯,在单因素实验的基础上,选择催化温度、酶添加量、水添加量作为自变量,1,3-甘油二酯产率作为响应值,利用Central Composite Design响应曲面设计方法,研究各反应条件对1,3-甘油二酯产率的影响。再利用Design Expert模拟二次多项式回归方程的预测模型,确定最佳条件组合。结果表明:在酶解温度为55℃,加水量30%,加酶量45%,1,3-甘油二酯在产率为56.82%,1,2-甘油二酯产率为12.45%,甘油二酯合计69.86%。

To optimize production condition of high purity 1,3-diacylglycerol from soybean oil hydrolyzed by immobilized Lipozyme TL IM, based on single factor experiments, the hydrolysis temperature, the amount of Lipozyme TL IM, and the amount of water were chosen as independent variables and the 1,3-diacylglycerol- formation rate was response, using the Central Composite Design response surface design method to study the variables and their mutual effect on the 1,3-diacylglycerol-formation rate.Then used the Design-Expert analysis, simulated quadratic polynomial regression equation prediction model, and determine the optimal combination conditions.The results showed that when the hydrolysis temperature was 5.5%,the amount of water was 30%, the amount of Lipozyme TL IM was 45% （of soybean oil） ,the 1,3-diacylglycerol-formation rate was 56.82% and the 1,2-dialylglycerol reached to 12.45% ,so the diacylglycerol-formation rate was 69.86%.