化橘红总黄酮纯化的工艺研究

Separation and purification of total flavonoids from Exocarpium citri Grandis

比较了5种大孔树脂对化橘红总黄酮的吸附和解吸特性,以吸附率、解吸率为考察指标,对大孔树脂纯化化橘红总黄酮的工艺进行研究。结果表明:D-312大孔树脂对化橘红总黄酮的吸附和解吸性能最好。最佳吸附工艺条件为:上样液pH4,吸附流速1.0mL/min;最佳洗脱条件为:pH7,洗脱流速1.0mL/min,洗脱液体积分数80%,经D-312大孔吸附树脂纯化后的化橘红总黄酮纯度由14.16%提高到53.5%。

Taking the absorbability and desorption properties as parameters, the purifying conditions of total flavonoids from Exocarpium citri Grandis by macroporous resins,compared with five types of macroporous resins was investigated.The result showed that D-312 macroporous resins was the best one.The optimal adsorption process conditions were as follows, pH4,1.0mL/min adsorption velocity.Besides,the best desorption conditions, pH7,L0mL/min desorption velocity of flow, concentration of desorption soluntion 80%. The purity of general flavonoids extracted from Exocarpium citri Grandis was raised from 14.16% to 53.5%.