响应面法优化龙须菜多糖提取工艺

Optimization of extraction conditions for polysaccharides from Gracilaria lemaneiformis by response surface methodology

采用响应面法对超声辅助提取龙须菜多糖的工艺进行优化。在单因实验基础上,以多糖提取率为响应值,根据Box-Behnken实验设计原理,通过响应面分析得到优化组合条件。结果表明,龙须菜多糖超声辅助提取的最佳工艺参数为:超声时间10min、提取功率100W、提取温度90℃和液料比120:1mL/g。在该工艺条件下多糖提取率预测值为29.7979%,验证值为29.8724%。四种不同方法对多糖的脱蛋白效果显示,胰蛋白酶法具有良好的脱蛋白效果,可以较好地脱除蛋白,并使多糖损失减少。

The extraction technology of polysaccharide from Gracilaria lemaneiformis by ultrasonic-assisted method was optimized using response surface methodology（RSM）.On the basis of single factor experiments,the yield of polysaccharide as response value,the optimized extraction conditions was obtained by designing a Box-Behnken central composite experiment and the method of response surface analysis.The optimal extraction conditions were as follows, extraction time of ]0min, ultrasonic power of ]00W, extractions temperature of 90~C and the ratio of material to liquid 120： ]ml_/g. Under optimal conditions, the predicted and experimental extraction yields of polysaccharides were 29.7979% and 29.8724%, respectively.The results of the comparison with four methods of deproteinization showed that the trypsin enzyme method was an effective deproteinization method, with higher removal of dissociated protein and lower loss of polysaccharide.