火棘果中原花青素含量测定方法的建立

Determination of proanthocyanidins content in Pyracantha fortuneana fruit

采用香草醛-盐酸法对火棘提取物中原花青素含量进行测定,考察了标准物、光照条件、反应时间、反应温度、盐酸浓度、香草醛浓度等因素对原花青素与香草醛显色反应的影响。结果表明,香草醛-盐酸法测定火棘提取物中原花青素含量的适宜条件为:1mL提取液,6mL4%香草醛甲醇溶液以及3mL浓盐酸,混匀后不避光条件下30℃水浴保温10min后,以儿茶素为标准品,波长500nm处测定吸光度值。该测定方法具有良好的重复性、稳定性、重现性,回收率较高。

Vanillin-HCl assay was adopted to determine the content of proanthocyanidins in Pyracantha fortuneana extract.Several parameters, such as standard, light, reaction time, reaction temperature l HCl concentration and vanillin concentration that affecting the color reaction of proanthocyanidin were investigated.The results showed the appropriate reaction conditions were as the following, 1 mL of pyracantha extract,6mL of 4g/100mL vanillin solution and 3mL of concentrated HCI in methanol respectively,after blending the reaction was carried out at 30℃ for 10min and then measured the absorbance at 500nm.Assessment of the method by statistics showed the method has good repeatability, stability, reproducibility and high recovery rate.