ATP生物发光法检测布拉氏酵母菌总数的研究

Study on ATP Bioluminescent method for the detection of total count of Saccharomyces boulardii

利用ATP生物发光法建立了布拉氏酵母菌与相对发光值的定量线性模型,对比研究了十六烷基三甲基溴化铵(CTAB)和苯扎溴铵(BAB)两种提取剂在不同的浓度、提取时间下对酵母菌相对发光值的影响。结果表明,浓度为0.015%CTAB作用时间4min的提取效果最好。同时,对检出的RLU值的对数值与菌落总数的对数值作统计学线性相关分析发现,R^2=0.9919,具有统计学意义。ATP生物发光法对布拉氏酵母菌的最低检测限是10~3cfu/mL。

In this paper,ATP Bioluminescence method was used to set up the quantitative linear model for total count of Saccharomyces boulardii and relative light unit.Comparative research was applied to study the effect of relative light unit of Saccharomyces boulardii in two kinds of extracting agent between the Cetyltrimethylammonium bromide（CTAB） and the Benzalkonium bromide （BAB）in different concentration and extracting time. Results showed the best concentration of CTAB was 0.015% and the optimum extracting time was 4min.Meanwhile, statistic method used to analyze the correlation between the logarithm value of RLU and total plate count showed R^2 = 0.9919,which was statistically significant.The detection limit of ATP Bioluminescence method for Saccharomyces boulardii could reach 10^3cfu/mL.