桃花多酚对抑郁小鼠海马单胺递质改变及干预作用

Effects of peach blossom polyphenol on 5-HT and levels of norepinephrine in hippocampus of depression mice

目的探讨桃花多酚对慢性应激诱导的抑郁小鼠干预作用及其对脑海马五羟色胺、去甲肾上腺素水平影响。方法将50只昆明小鼠根据体重和1%蔗糖水偏嗜度随机分成5组,即模型对照组,氟西汀组,桃花多酚低、中、高剂量组(20、40、80 mg/kg);利用慢性轻度不可预见性刺激制造抑郁动物模型,观察模型小鼠行为改变及海马组织五羟色胺、去甲肾上腺素含量。结果模型组,氟西汀组,低、中、高桃花酚组小鼠逃避失败次数分别为(18.60±2.72)、(3.20±1.23)、(15.10±2.23)、(7.00±2.67)、(4.70±2.06)次;小鼠悬尾不动时间分别为(13.20±2.90)、(3.90±1.20)、(9.00±2.31)、(5.90±2.51)、(4.20±1.40)s;与模型组比较,桃花多酚各剂量组小鼠逃避失败次数降低且小鼠悬尾不动时间缩短,中、高剂量组均优于低剂量组(P<0.01);桃花多酚各剂量均能升高脑海马五羟色胺和去甲肾上腺素含量(P<0.01),与氟西汀具有相似抗郁效果。结论桃花多酚能明显增加脑海马五羟色胺和去甲肾上腺素含量,其抗抑郁作用机制可能与之有关。

Objective To assess the effects of peach blossom polyphenol（PBP） and levels of norepinephrine（NE） and 5-hydroxytryptamine（5-HT） in hippocampus of mice with chronic depression induced by unpredictable mild stress. Methods Based on body weight and addiction to 1% sucrose water,50 Kuming mice were randomly divided into model control group, fluose fine hydrochloride （FFH） group, three PBP treatment groups at doses of 20 mg/kg, 40 mg/kg, and 80 mg/kg. The depression model of the mice was induced by chronic unpredictable mild stress. The changes in behavior and hippocampus 5-HT,NE content of the mice were observed and compared among different groups. Results Com- pared with model group, the frequency of avoidance failure and immobility time of tail suspension in PBP treatment goups were significantly reduced with a dose-response manner（ P 〈 0. 01 ）. PBP treatment significantly increased brain 5-HT and NE levels （P 〈 0. 01 ） with an anti-depression effect similar to that of FFH. Conclusion PBP can significantly increase brain 5-HT and NE levels, which could be the mechanism of antidepressant effect of PBP.