一对母婴同时携带的金黄色葡萄球菌同源性分析

Analysis on homology of Staphylococcus aureus carried by a mother and her newborn

目的分析一对母婴同时携带的金黄色葡萄球菌的同源性,了解金黄色葡萄球菌在母婴之间传播的可能性。方法对分离的3株金黄色葡萄球菌(其中1株分离自受母乳喂养,患有脐炎的新生儿脐分泌物;另2株分别分离自该新生儿健康母亲左右两侧乳房分泌的乳汁)进行药敏试验表型分型和脉冲场凝胶电泳(PFGE)基因分型,分析其同源性。结果此对母婴携带的3株金黄色葡萄球菌对常用抗菌药物的药敏试验结果完全相同,PFGE图谱具有100%的相似度,PFGE结果聚类图处于同一分支。结论此对母婴携带的金黄色葡萄球菌是相同型别菌株,推测金黄色葡萄球菌在母婴之间传播的可能性大。

Objective To study the homology of Staphylococcus aureus （S. aureus） isolated from a mother and her newborn, and to explore the possibility of transmission of S. aureus infection between mother and newborn. Methods Three S. aureus isolates from the umbilical secretion of a newborn with omphalitis and from the breast milk of the newborn＇s healthy mother were performed antimicrobial susceptibility testing,homology of S. aureus was analyzed through antimicrobial susceptibility testing phenotyping and pulsed-field gel electrophoresis （PFGE） genotyping. Re- suits Three S. aureus isolates from the mother and newborn had the same antimicrobial susceptibility pattern and PFGE type. Conclusion S. aureus isolated from the mother and newborn were identical strains, suggesting a possi- bility of S. aureus transmission between mother and newborn.