血清辛伐他汀HPLC/ESI/MS和FIA/ESI/MS定量测定方法

Determination of Simvastatin by HPLC/ESI/MS and FIA/ESI MS

本文以电喷雾为接口的液质联机对血清中的降血脂药物辛伐他汀进行了HPL C/ESI/MS和流动注射分析的 FIA/ESI/MS定量测定的方法学研究。样品以 C- 8固相萃取柱为主要前处理手段 ,血清中的加样回收率为 88%～ 1 0 1 %。应用 HPL C/ESI/MS,方法绝对检出量为 0 .3ng,检出极限为 0 .5ng/ml(血清 )。内标校正曲线的线性拟合 r2 =0 .996,应用 FIA/ESI/MS线性拟合 r2 =0 .

This paper reports a new method for determination of simvastatin in serum ,which is a regulator of blood fat.The sample serum is treated by solid phase extraction,and the recovery of the drug is about 90%.With HPLC/ESI/MS the detectable limit is 0.5ng/ml(serum).The correlation coefficient of internal calibration is r 2=0.996.For FIA/ESI/MS the coefficient of external calibration is r 2=0.986.