不同制备工艺对防感喷雾剂药效学的影响

Pharmacodynamics Research of Different Preparations of Fanggan Spray

目的:通过简单、快捷的实验初步评价防感喷雾剂几种工艺样品的体外抗菌、镇痛、抗炎活性,为制备工艺筛选提供实验依据。方法:采用平皿钢圈法、小鼠醋酸扭体和二甲苯致耳廓肿胀实验,观察比较各样品的抗菌、镇痛和抗炎作用。结果:以金黄色葡萄球菌作为质控菌评价,其作用顺序为:青霉素钠>FG-4>FG-3>FG-1>FG-2;以同等生药剂量连续灌胃给药3 d,对小鼠醋酸致痛的抑制作用顺序为:ASP组>蓝芩口服液组>FG-1组≈FG-3组>FG-4组>FG-2组>双黄连口服液组;二甲苯致耳廓肿胀实验抑制作用顺序为:FG-3组>ASP组>FG-1组>FG-2组>FG-4组>双黄连口服液组>蓝芩口服液组。结论:新工艺FG-3样品不但保留原工艺FG-4样品的抗菌、镇痛、抗炎作用,且均有所增强,为防感喷雾剂的工艺筛选及改进提供了部分实验依据。

Objective： To evaluate different simples of Fangan spray in antibacterial action in vitro, analgesia and anti-inflammatory, and provide the evidence to the preparation process of screening. Methods： Antibacterial action, analgesic and anti-inflammatory effects of each sample were observed and compared by Petri dish and rims method, mouse writhing test and xylene induced ear edema test. Results： Using staphylococcus aureus as quality evaluation strain, the effect sequence was Sodium penicillin〉FG-4〉FG-3 〉FG-1 〉FG-2. After three-day＇ administration with the same doses, the effect of pain relief sequence was ASP〉Lanqin oral liquid〉FG-1 ~ FG-3〉FG-4〉 FG-2〉Shuanghuanglian oral liquid. The anti-inflammatory effect sequence was FG-3 〉ASP〉 FG-1 〉 FG-2 〉 FG-4 〉Shuanghuanglian oral liquid〉Lanqian oral liquid. Conclusions： Comparing with FG-4, the antibacterial action in vitro, analgesis and anti-inflammatory effects of FG-3 were strengthened. FG-3 provides some experimental basis for screening and improvement of Fangan spray preparations.