胰岛素受体底物-1基因多态性与多囊卵巢综合征

Association between the Polymorphism of the Insulin Receptor Substrate-1 Gene and Polycystic Ovary Syndrome

目的 通过检测多囊卵巢综合征 (PCOS)病人的胰岛素敏感指数等指标 ,观察是否存在胰岛素抵抗 ,对 PCOS病人胰岛素受体底物 - 1(IRS- 1)基因多态性进行分析 ,探讨 IRS- 1基因与 PCOS的关系 ,判断其可否作为PCOS胰岛素抵抗的致病基因。 方法 采用 PCR技术检测正常人与 PCOS病人 IRS- 1的等位基因与基因频率 ,据不同的基因型 (GG、GA或 AA)将病人分为 3组 ,比较各组的体重指数、腰臀围比例、黄体生成素、游离睾酮、雄烯二酮、胰岛素敏感指数的水平。 结果 多囊卵巢综合征不同的基因型各生化指标差异无显著性 ,但确实存在胰岛素抵抗 ,胰岛素敏感指数可较好地反映其程度。 结论 PCOS的发病与胰岛素受体底物 - 1基因变异无明显关系。

Objective\ To analyse insulin sensitive index(IAI) as a marker indicating insulin resistance(IR) which is significant in polycystic ovary syndrome(PCOS).\ To investigate the polymorphism of the insulin receptor substrate-1 gene(IRS-1),and estimate whether IRS-1 gene is a pathogenic gene.\ Methods\ The polymorphism of IRS-1 was detected by PCR technique,patients were divided into three groups according to genotype.\ Body weight index(BMI),waist-hip ratio(WHR),luteinizing hormone(LH),free testosterone(FT),androstanedione(A\-2) and insulin active index(IAI) were detected and compared among them.\ Results\ IAI was better to mirror the degree of insulin resistance in polycystic ovary syndrome(PCOS).\ There were no difference with BMI,WHR,LH,FT,A\-2 and IAI among various genotype groups in the patients with PCOS.\ Conclusion\ IR in PCOS is not significantly associated with the variant of the IRS-1 gene.\;