人参总皂苷对β-淀粉样肽致小鼠记忆障碍的影响

Effect of total ginsenoside on memory impairment induced by β amyloid peptide

目的 研究人参总皂苷对 β 淀粉样肽 (β AP)所致学习记忆障碍的影响。方法 给小鼠一次性icv凝聚态 β AP活性片段 (2 5～ 35 ) 3μl (1 0mmol·L-1)造成早老性痴呆动物模型 ,采用避暗试验及Morris水迷宫试验 ,观察人参总皂苷对 β AP(2 5～ 35 )神经毒性的对抗作用。 结果 一次性icv凝聚态 β AP(2 5～ 35 )能导致学习记忆障碍。人参总皂苷(10 0 ,5 0 ,2 5mg·kg-1,ig)可明显改善 β AP(2 5～ 35 )所致被动回避性记忆障碍和空间记忆障碍。结论 人参总皂苷可对抗 β AP(2 5～ 35 )的神经毒性 ,改善 β AP(2 5～ 35 )

AIM To observe the effect of total ginsenoside on learning and memory impairment induced by β amyloid peptide(β AP). METHODS Aggregated β AP(25～35) 3 μl (1 0 mmol·L -1 ) icv once to mice was used as an AD animal model. In step through test and Morris water maze test, the effects of total ginsenoside ig once a day at doses of 100,50,25 mg·kg -1 on β AP(25～35) induced memory impairment were observed. RESULTS In mice, aggregated β AP(25～35) induced obvious learning and memory impairment in step through test 8 days and in Morris water maze test 11 days after icv β AP(25～35). Total ginsenoside 100,50,25 mg·kg -1 significantly improved memory impairment induced by β AP(25～35). CONCLUSION The β AP(25～35) icv can impaire memory. Total ginsenoside improves memory impairment induced by aggregated β AP(25～35).