肝康颗粒对肝纤维化大鼠肝组织及血清PCⅢLN影响的实验研究

Liver Fibrosis Kang Granule on Rat Liver Tissue and Serum PCⅢ,LN Influence Experimental Research

目的:通过观察中药复方肝康颗粒对肝纤维化(Hepatic Fibrosis)大鼠肝组织Ⅲ型前胶原(Precollagen TypeⅢ)层粘连蛋白(Laminin)表达以及血清PCⅢ、LN含量的影响,探讨肝康颗粒抗肝纤维化的治疗效果和其作用机制。方法:选取SD雄性大鼠皮下注射四氯化碳(CCl4)植物油溶液和自由饮用酒精的方法制备肝纤维化大鼠模型,检测大鼠血清Ⅲ型前胶原、层粘连蛋白的含量;显微镜下观察肝组织的变性程度及肝纤维化程度;用免疫组化方法检测肝组织PCⅢ、LN的表达。结果:肝康颗粒能显著降低肝纤维化大鼠的血清PCⅢ、LN含量,减低肝组织炎症活动度及纤维化程度。PCⅢ的检测结果表明:与正常对照组比较,病理模型组肝组织中Ⅲ型胶原明显增生,具有显著性差异(P<0.01);与病理模型组比较,肝康颗粒大剂量、肝康颗粒中剂量、大黄蛰虫丸三个治疗组肝组织中Ⅲ型胶原含量明显减少,有显著性差异(P<0.05)。LN的检测结果表明:正常对照组大鼠肝组织LN含量很低;病理模型组大鼠肝组织层粘连蛋白含量与正常对照组相比明显增多,具有显著性差异(P<0.01);肝康颗粒大剂量、中剂量、大黄蛰虫丸三个治疗组与病理模型组比较,肝组织中LN含量明显减少,有显著性差异(P<0.05)。结论:肝康颗粒可明显降低肝纤维化大鼠肝组织及血清中PCⅢ、LN的含量,有效减轻大鼠肝组织炎症程度及纤维化程度。

Objective： Through the observation of traditional Chinese medicine compound liver kang granule on liver Fibrosis （Hepatic Fibrosis） rat liver tissue before Type III collagen （Precollagen Type IlI） Laminin （Laminin） expression and serum PCIII, LN content, this paper discusses the influence of liver kang grain resistance of liver Fibrosis treatment effect and its mechanism of action 1. Methods： The SD male rats subcutaneous injection of carbon tetrachloride （CC14） vegetable oil solution and free drinking alcohol preparation method of liver fibrosis rats model, testing rats serum collagen type III before,laminin content; under a microscope to observe the liver tissue degeneration degree and the degree of liver fibrosis,with immunohistochemical method detection liver tissue PC III, LN expression. Results：The liver kang particle can significantly reduce serum liver fibrosis rats PC III, LN, reduce the content of liver tissue inflammation activity and the degree of fibrosis. PC III testing results show that the normal control group and model group compared, pathology of liver tissue collagen type III obvious hyperplasia,with significant difference （ P 〈0.01）;And the pathologic model group comparison, liver kang particle large dose,liver kang grains dose,rhubarb worm pill three of its treatment group in the liver tissue III type collagen content significantly reduced,there is significant difFerence（ P 〈0. 05）. LN testinjz results show that the normal control group rat liver tissue LN content is very low; The pathologic model group rat liver tissue laminin content and compared with normal control group significantly increased, with significant difference（ P 〈0.01） ;Liver kang particle large dose,dose, rhubarb worm pill three of its treatment group and pathologic model group comparison,liver tissue LN content significantly reduced,there is significant difference（ P 〈 0.05）. Conclusion： Liver kang granule can obviously decrease the liver fibrosis rat liver tissue and serum PC III, LN content, effectively reduce rat liver tissue inflammation degree and the degree of fibrosis.