特发性中枢性性早熟女童血清Kisspeptins水平的研究

Research on serum Kisspeptins in Chinese girls with idiopathic central precocious puberty

【目的】通过检测Kiss肽(Kisspeptins)在健康女童及特发性中枢性性早熟(idiopathic central precociouspuberty,ICPP)女童治疗前后血清中的水平变化,探讨其在ICPP发生过程中的作用,及对诊断及治疗该病的意义。【方法】以20名6～8岁TannerⅠ期健康女童作为健康对照组,经生长发育评价及相关检查选取20名6～8岁处于TannerⅡ期的ICPP女童作为实验组,对实验组女童进行促性腺激素拟似物(gonadotropin releasing hormone analogues,GnRHa)治疗6月;用免疫化学发光法测血清中黄体生产素(luteinizing hormone,LH)水平;Western Blot法检测女童血清中Kisspeptins水平。【结果】治疗前实验组血清Kisspeptins水平较健康对照组增加了0.86倍(P<0.01),治疗后实验组血清Kisspeptins水平较治疗前降低了0.97倍(P<0.01);治疗前实验组血清Kisspeptins水平与LH峰值、骨龄均存在正相关,(r=0.42和0.38,P<0.05);治疗后实验组血清Kisspeptins水平与LH峰值、骨龄不具有明显相关性(r=0.05和0.09,P>0.05)。【结论】Kisspeptins/G蛋白偶联受体54(G protein-coupled receptor 54,GPR54)信号在女童特发性中枢性性早熟启动过程中起重要作用,血清Kisspeptins水平对诊断及治疗特发性性早熟具有指导意义。

[Objective] To explore the role of Kisspeptins in the onset of idiopathic central precocious puberty and re-alize the significance about diagnosis and treatment by detecting the level of Kisspeptins in blood serum. [Methods] A total of 40 girls aged 6-8 years old were divided into 2 groups by assessment of growth and development and correlated ex-aminations：20 healthy girls of Tanner Ⅰ in control group, 20 ICCP girls of Tanner Ⅱ in study group, ICCP girls were treated with GnRHa for six month. And then the levels of luteinizing hormone by Chemiluminescence and Kisspeptins were detected by Western Blot. [Results] Compared with the control group,the level of Kisspeptins in study group before treatment in-creased 0.86 fold （P〈0.01） ; compared with the study group before treatment, the level of Kisspeptins in study group after treatment decreased 0.97 fold （P〈0.01）;the level of Kisspeptins in study group before treatment was positive correlated with both the peak value of LH and their bone ages individually, the Spearman correlation coefficients were 0.42 and 0.38（P 〈0.05） ;the level of Kisspeptins in study group after treatment was not correlated with both the peak value of LH and their bone ages individually,the Spearman correlation coefficients were 0.05 and 0. 09（P〉0. 05）. [Conclusion] Kisspeptins/ GPR54 system may play an important role in the onset of idiopathic central precocious puberty, the level of Kisspeptins in blood serum has a guiding significance for diagnosing and treating idiopathic central precocious puberty.