γ-维生素E对哮喘小鼠血清及肺泡灌洗液嗜酸性粒细胞趋化因子的影响

Effect of gamma-tocopherol on eotaxin of asthmatic mice

【目的】探讨γ-维生素E(γ-T)对哮喘的治疗作用及机制。【方法】将40只雄性Balb/c小鼠(4周,18～20g)随机分为正常组、哮喘组、地塞米松组及γ-T治疗组。给予小鼠腹腔注射及雾化吸入卵白蛋白建立哮喘气道炎症反应模型,分别给予地塞米松及γ-T,正常组仅给予生理盐水。28d后处死小鼠,留取血清及肺泡灌洗液。ELISA法测定各组血清及肺泡灌洗液中嗜酸性粒细胞趋化因子(eotaxin)的含量。用one-way ANOVA检验组间差异,P<0.05具有统计学意义。【结果】哮喘组小鼠血清及肺泡灌洗液中eotaxin浓度均较正常组升高,地塞米松组及γ-T组均较哮喘组下降。在血清中地塞米松组下降最明显,γ-T组次之,γ-T组与哮喘组,γ-T组与地塞米松组相比差异无统计学意义(P>0.05);在BALF中,γ-T组的eotaxin下降最明显,与哮喘组相比,二者间差异明显(P<0.05);γ-T组与地塞米松组相比,二者差异无统计学意义(P>0.05)。【结论】γ-T有利于减少哮喘小鼠肺组织eotaxin,γ-T可能是有用的抗哮喘药物。

[Objective] To explore the effect and mechanism of gamma-tocopherol（γ-T） in asthmatic mice. [Meth-ods] A total of 40 male Balb/c miee（4weeks,18-20 g） were randomly divided into four groups：asthma group,dexametha-sone group, γ-T group, and normal group. The mice of asthma group were received intraperitoneal injection of OVA （10 μg） on day 0,γ,14 and exposed to 25 g/L OVA inhalation from day 21 for one week. Ovalbumin （OVA）-sensitized mice were challenged with dexamethasone and γ-T for one week, the normal group were only given saline. Blood plasma and BALF samples were collected after the 28 d mice were sacrificed. The concentration of eotaxin in serum and BALF were determined by the method of ELISA. One-way ANOVA was used test the difference between groups. [Results] The concentration of eotaxin in serum and BALF of asthmatic mice were higher than normal, and those were lower in dexamethasone and γ-T group. The concentration of eotaxin in serum of γ-T group were lower compared with asthma and dexamethasone group（P〉 0.05） ;The concentration of eotaxin in BALF of γ-T group were lower compared with dexamethasone and asthma group（P all〈0.05）. [Conclusion] γ-T prohibit the expression of eotaxin in asthmatic mice,and γ-T might be an effective therapy of allergic airway inflammation.