亚临床酮病对奶牛产后子宫恢复状况和血液白细胞的影响

Effects of Subclinical Ketosis on Uterine Recovery and White Blood Cell Count in Postpartum Dairy Cows

根据胎次、年龄、产奶量、分娩日期分别筛选出亚临床酮病奶牛、非酮病奶牛各22头作为试验组、对照组奶牛;于分娩后第15天,使用兽用B型超声诊断仪对试验奶牛进行子宫颈、子宫角直径的测量,并采集血样进行白细胞分类计数。于分娩后第40天,对试验奶牛进行子宫颈黏液采样、涂片、染色,镜检多形核白细胞的浸润情况。结果显示,与对照组相比,分娩后15d试验组奶牛的子宫颈直径要显著增大(P<0.05),白细胞总数(WBC)、淋巴细胞数量(LYM)和中性粒细胞数量(NEU)显著降低(P<0.05)。分娩后40d时,试验组奶牛子宫颈黏液中各评定等级下的多形核白细胞(PMN)浸润率均较对照组奶牛高,两组间差异不显著(P>0.05)。试验表明,亚临床酮病奶牛的子宫复旧出现了一定程度的推迟,子宫更容易出现炎症反应。

Based on the similarity in age, parity, milk yield, date of parturition, 22 cows with subclinical ketosis were used as the test group and same number of healthy cows without ketosis were applied in control group. 15 days after parturition, the diameter of cervix and uterine horn were measured with B- mode ultrasonography. Blood specimens were collected for WBC differentiation test. 40 days after parturition,cervical mucus samples were collected and smears prepared. After the staining of the smears, the morphological studies of cervical specimen were conducted to determine the polymorphonuclear leukocyte. The result indicated that the diameters of cervix in test group were significantly larger than control group （P〈0. 05）. Immunocytes including WBC, LYM and NEU, were significantly lower in the test group （P〈0.05）. Based on the standard evaluation system, the rate of infiltrated PMN in the cervix mucus of test group was higher than that of control group. However, the difference of this index between two groups was not significant （P〉0. 05）. This study indicated that the uterine recovery in the dairy with subclinical ketosis was obviously delayed,leading to the higher susceptibility of uterine inflammation.