不结球白菜雄性不育新种质及其保持系的叶绿素荧光参数比较

Comparison of Chlorophyll Fluorescence Parameter between a New Male Sterile Germplasm and its Maintainer Line in Pak-choi

利用叶绿素荧光技术,在4个不同时期分别测定不结球白菜雄性不育新种质及其保持系的各叶绿素荧光参数,以及叶绿素的SPAD值。结果表明:不育系在生长的不同时期,叶绿素含量的变化与保持系一致,且差异不显著;初始荧光Fo、最大光化学效率(Fv/Fm)、实际光化学效率(ΦPSⅡ)、光化学淬灭系数(qP)和非光化学淬灭系数(qN),不育系与保持系在各时期的变化趋势一致,但在抽薹期和开花期存在差异,表明该不结球白菜的不育系与保持系在营养生长过程中植物学性状一致,生殖生长期的差异可能与其不育性有关。

The chlorophyll fluorescence parameters and the content of chlorophyll SPAD both in male sterile germplasm in Pak-choi （Brassica campestris ssp. chinensis Makino L. ） and its maintainer line were measured by chlorophyll fluorescence technique in four different periods. The results showed that the changes of chlorophyll SPAD in the male sterile line is similar with its maintainer in different growth periods. These parameters including the initial fluorescence Fo, maximum photochemical efficiency （Fv/Fm）, actual photochemical efficiency （ФPSⅡ）, photochemical quenching coefficient （ qP ）, and non-photochemical quenching coefficient （qN） demonstrated similar changing trends in sterile strain and maintainer in different growing period. However, during bud and blossom period, these indexes showed obvious differences,indicating that the new germplasm from male sterile line had the same botany character with its maintainer line in vegetative growth period. The difference in reproductive growth was associated with male sterility.