包装内CO_2含量对冷却肉特征微生物生长的影响

Effect of CO_2 Concentration in Packaging on the Growth of Microorganism from Chilled Meat

将来自冷却猪肉的混合菌种接种于液体培养基中,采用不同CO2含量气调包装后,于5℃下贮藏。采用相应的选择性培养基测定其中假单胞菌属、肠杆菌科及乳酸菌3种特征微生物的生长规律,同时测定了菌落总数的变化。结果表明:兼性厌氧型微生物乳酸菌对气调包装内CO2含量变化不敏感;气调包装内CO2对需氧微生物肠杆菌科微生物和假单胞菌属的最大比生长速(μmax)存在抑制作用,CO2含量越大,抑制作用越强;样品中菌落总数的变化也受到CO2含量的影响,高浓度CO2会抑制菌落总数的增长速率;CO2含量的变化对肠杆菌科和假单胞菌属生长速率及菌落总数的增长速率的影响,可以通过一个2参数平方根方程进行很好地描述。

The mixed strains from chilled pork were inoculated into the liquid medium which was packaged with different concentration of CO2. The samples were stored at 5 ℃ and the number of Pseudomonas, Enterobacteriaceae, Lactic acid bacteria and total number colonies were collected at different time during the storage. The results demonstrated that the concentration of CO2 has little influence on Lactic acid bacteria since its facultative anaerobes ; CO2 concentration can effectively curb the maximal growth speed of Pseudomonas and Enterobacteriaceae ; the higher the CO2 concentration, the stronger the inhibition ; high CO2 concentration can curb the growth speed of total number of bacteria colony; the inhibition effect of CO2 concentration on the growth speed of Pseudomonas and Enterobacteriaceae and the growth speed of total number of bacteria colony can be described by a two-parameter equation.