高效液相色谱-蒸发光散射检测法测定加味益气丸中黄芪甲苷的含量

Determination of Evaporative Light Scattering Detection by High Performance Liquid Chromatography Content of Jiawei Yiqi Pill Astragaloside

目的:建立加味益气丸中黄芪甲苷含量的测定方法。方法:采用高效液相色谱-蒸发光散射检测(HPLC-ELSD)法,以乙腈:水(32∶68)为流动相,流速为1.0 mL·min-1;漂移管温度为98℃,氮气流速为1.5 L·min-1。结果:黄芪甲苷在0.852～5.112μg范围内线性关系良好(r=0.999 2),平均回收率为96.28%,RSD为1.18%(n=5)。结论:本研究建立的HPLC-ELSD法专属性强、准确度高、重复性好,可用于加味益气丸的的黄芪甲苷的含量测定。

Objective： To estabish the method for determination of content of astragaloside in Jiawei Yiqi Pill. Methods： Using high performance liquid chrimatography with evaporative light scattering detection method, acetonitrile：water （32 ： 68） as the mobile phase, the flow rate was 1.0 mL · min^（-1） ; the drift tube temperature was 98 degrees celsius, the nitrogen flow rate was 1.5 L · min^（-1）. Results ： Astragaloside good linear relation within O. 852 - 5.112 μg（ r = 0.999 2）, the average recovery was 96.28%, RDS was 1. 18% （ n = 5 ）. Conclusion：The HPLC-ELSD method established in this study with strong specificity, high accuracy, good repeatability,can be used for determination of content of astragaloside in Jiawei Yiqi Pill.