Eu-TTA体系测定条件的优化及其在小麦代谢研究中的应用

Study of Eu(III) Metabolism in Wheat Cells by Eu(III) - TTA -4,7- Diphenyl -2,9- dimethyl - 1,10 - phenanthroline System

研究了铕(Ⅲ)-TTA(邻-A噻吩甲酰三氟丙酮)体系在有机溶剂和邻菲啰啉衍生物存在下的荧光性质,发现醇类有机溶剂能使Eu(Ⅲ)-TTA体系荧光强度大幅度提高;三种邻菲啰啉衍生物中,4,7-二苯基-2,9-二甲基-1,10-邻菲啰啉的加入也能提高体系灵敏度,优化了反应条件,并用此法研究了小麦根施Eu^(3+)数天后,Eu^(3+)在根和叶片细胞膜和细胞内的分布定位,为稀土元素的植物代谢研究提供了一种重要的辅助研究手段.

Study on the fluorescence properties, the application in plant metabolism and distribution and localization of Eu( III ) - TTA system has been undertaken. The presence of alcohol solvent and 4,7- diphenyl -2,9- dimethyl - 1,10- phenanthroline can yield high sensitivity. The measuring conditions have been improved and used satisfactorily in analyzing the content of Eu (III) which was absorbed by wheat (triticum. aestivum L) root and leave cells. An optimal method has been developed for the study of plant metabolism.