摘要
目的:山梨醇脱氢酶具有不完全氧化多种羟基化合物的特性,在工业中已广泛应用。该文研究该酶的酶学性质。方法:利用单因素实验对山梨醇脱氢酶的储藏稳定性和操作稳定性、反应液的pH和反应温度对酶活力的影响进行了研究,并进行了反应动力学研究。结果:反应液的pH值为5.5、反应温度为28℃时,酶活力最高;单批次反应过程中,酶基本没有失活。底物耗尽时,连续进行两次补加相同量的底物,酶活力有较小的降低。第一次重复时,酶活力有较小的降低;在第二次重复使用时,丧失40%的活力;山梨醇脱氢酶的底物特异性常数Ks为51 mmol/L,单位生物量的山梨醇脱氢酶最大活力Umax为1.7U/mg干细胞。结论:山梨醇脱氢酶的稳定性较高。
Objective: Sorbitol dehydrogenase has the characteristic of non - complete oxidating polyols, and it has been applied in industry extensively. Enzymatic characteristics were studied in the article. Method: Storing and using stability, pH and temperature of reactive liquid and kinetic of sorbitol dehydrogenase were studied by single experiment. Result: When pH was 5.5 and temperature was 28, enzyme activi- ty was the hightest. In the course of usage, enzyme activity almost did not fall. When substrates were used up and the same amount of sub- strates were supplied continuously, enzyme activity lost little. When it was used again, enzyme activity lost little. When it was used in the third time, enzyme activity lost 40%. Ks and Umax was 51 mmol/L and 1.7U/mg dry cell respectively. Conclusion:Stabilities of sorbitol dehydrogenase were high.
出处
《生物技术》
CAS
CSCD
北大核心
2013年第1期70-73,共4页
Biotechnology
关键词
山梨醇脱氢酶
酶活力
酶活稳定性
sorbitol dehydrogenase
enzyme activity
enzyme stability