摘要
目的:研究茅苍术提取物对肺癌A549细胞的肿瘤增殖抑制效果及机制。方法:MTT比色法检测茅苍术提取物对A549细胞增殖的抑制效果;流式细胞术分析茅苍术提取物对肺癌A549细胞周期的影响;Western Blot检测茅苍术刺激前后细胞周期蛋白D1(cyclin D1)蛋白表达量变化。结果:茅苍术提取物能有效抑制肺癌A549细胞增殖,且抑制效果与茅苍术提取物浓度呈现依赖性,茅苍术提取物刺激48h后,抑制效果最佳,IC50约为77.12μg/ml;细胞周期分析显示:茅苍术提取物可将细胞周期阻滞在G0/G1期,50μg/ml茅苍术提取物刺激48h后,G1期上升至53.41%;cyclin D1蛋白含量因茅苍术作用而下调。结论:茅苍术提取物能通过有效抑制cyclin D1的表达,进而将细胞周期阻滞于G1期,最终实现其抗增殖作用机制。
Objective :To research the anti - proliferation effect of extract of Rhizoma Atractylodis on A549 cells. Method:Rhizoma Atrac- tylodis was extracted by 90% ethanol using ultrasonic method for l h, then supernatant was collected by centrifugation; The proliferation activity of A549 inhibited by extract of Rhizoma Atractylodis was measured by MT^F assay. The effect of extract of Rhizoma Atractylodis on cell cyclic was detected by flow eytometry; cyclin D1 protein expression was measured by Western Blot. Result: Extract of Rhizoma Atrac- tylodis can effectively inhibit the proliferation of A549 in dosage and time dependent manner, the best inhibitory effect was appeared on 48h, the ICso is about 77. 12pLg/ml; extract of Rhizoma Atractylodis can block cell cyclic in GO/G1 phase, the G1 phase was increased to 53.41% when A549 cells inhibited for 48h and effectively decrease cyclin D1 protein expression. Conclusion: Rhizoma Atractylodis can block cell cyclic in G0/GI phase via decrease cyclin D1 protein to realize anti -proliferation effect.
出处
《生物技术》
CAS
CSCD
北大核心
2013年第1期73-76,共4页
Biotechnology
关键词
茅苍术
肺癌
增殖
细胞周期蛋白D1
Rhizoma Atractylodis
lung cancer
proliferation
cyclin D1