通腑汤对大鼠快速胃排空影响的实验研究

Effects of Tongfu Decoction on Rats′ Rapid Gastric Emptying

目的观察通腑汤对正常大鼠胃排空的影响,探讨其有无快速促进胃排空作用。方法 30只SD大鼠随机分为正常对照组、吗丁啉组和通腑汤组,每组10只,分别予生理盐水、吗丁啉混悬液和通腑汤煎剂灌胃,30min后,采用以99mTc-DTPA标记的单光子发射计算机体层摄影技术(single photon emission computed tomography,SPECT)检测大鼠胃排空,计算胃半排空时间和胃排空率。结果正常对照组、吗丁啉组和通腑汤组的胃半排空时间分别为(19.0±1.7)min、(12.9±3.4)min、(12.7±4.1)min;与正常对照组比较,吗丁啉组和通腑汤组胃半排空时间均明显缩短(P<0.05)。3组15min胃排空率分别为(41.1±5.8)%、(52.9±10.9)%、(56.0±10.3)%,30min胃排空率分别为(65.6±2.8)%、(72.9±2.6)%、(72.4±4.9)%;与正常对照组比较,吗丁啉组和通腑汤组15、30min胃排空率均明显提高(P<0.05)。两用药组胃半排空时间和胃排空率比较,差异均无统计学意义(P>0.05)。结论通腑汤具有与吗丁啉类似的快速的促胃排空作用,并能缩短正常大鼠胃半排空时间。

Objective To observe the effects of Tongfu Decoction （TFD） on the gastric emptying of normal rats, thus exploring whether it could promote gastric emptying rapidly. Methods Thirty Sprague- Dawley （SD） rats were randomly divided into 3 groups, i.e., the normal control group, the domperidone group, and the TFD group, 10 in each group. They were respectively administered with normal saline, the domperidone suspension, and TFD by gastrogavage. Thirty min later the gastric emptying of mice was de- tected by single photon emission computed tomography technology （SPECT） labeled with 99m Tc-DTPA, and the gastric half-emptying time and the gastric emptying rate were calculated. Results The gastric half- emptying time was （19.0±1.7） min in the normal control group, （12.9±3.4） min in the domperidone group, and （12.7±4.1） min in the TFD group. Compared with the normal control group, the gastric half- emptying time was significantly shortened in the domperidone group and the TFD group （P 〈0.05）. The gas- tric emptying rate at 15 min was 41.1% ±5.8% in the normal control group, 52.9% ±10.9% in the domperi- done group, and 56.0%±10.3% in the TFD group, while at 30 min it was 65.6%±2.8%, 72.9%±2.6%, and 72.4%±4.9%, respectively. Compared with the normal control group, the gastric emptying rate at 15 min and 30 min both significantly increased in the domperidone group and the TFD group （P 〈0.05）. There was no statistical difference in the gastric half-emptying time or the gastric emptying rate between the two groups （P 〉0.05）. Conclusion TFD showed similar effects as domperidone in rapidly promoting gastric emptying, and could shorten the gastric half-emptying time in normal rats.