脂质体阿霉素和热疗对脑胶质瘤细胞的影响

Effects of liposome-adriamycin(L-ADM) and thermotherapy on glioma cells: an experimental study

目的探讨脂质体阿霉素和热疗对脑胶质瘤细胞的增殖抑制及凋亡诱导作用。方法体外培养人脑胶质瘤细胞SWO-38,采用水浴加温法,观察单纯热疗、阿霉素化疗、脂质体阿霉素化疗、热疗+阿霉素化疗、热疗+脂质体阿霉素化疗对细胞增殖及凋亡的影响。MTT法确定阿霉素和脂质体阿霉素的工作浓度并检测细胞增殖情况,流式细胞术检测细胞凋亡情况。结果热疗+脂质体阿霉素化疗组、热疗+阿霉素化疗组的增殖抑制率分别为80.16%±3.78%、62.09%±3.05%,均显著高于脂质体阿霉素化疗组(40.27%±2.32%)、阿霉素化疗组(30.56%±2.03%)、单纯热疗组(16.51%±1.26%),差异有统计学意义(P<0.05),且热疗+脂质体阿霉素化疗组的增殖抑制率显著高于热疗+阿霉素化疗组(P<0.05)。热疗+脂质体阿霉素化疗组、热疗+阿霉素化疗组的细胞凋亡率分别为84.19%±2.69%、60.29%±1.47%,均显著高于脂质体阿霉素化疗组(46.72%±2.09%)、阿霉素化疗组(35.09%±1.46%)、单纯热疗组(17.85%±0.78%),差异有统计学意义(P<0.05),且热疗+脂质体阿霉素化疗组的细胞凋亡率显著高于热疗+阿霉素化疗组(P<0.05)。结论加热可增强阿霉素及脂质体阿霉素对人脑胶质瘤细胞SWO-38的增殖抑制和凋亡诱导作用,且对脂质体阿霉素的增敏作用更强。

Objective To observe the effects ofliposome-adriamycin （L-ADM） and thermotherapy on proliferation and apoptosis ofSWO-38 glioma cells. Methods The SWO-38 glioma cells were cultivated in vitro. The effects ofthermotherapy （43℃）, ADM chemotherapy, L-ADM chemotherapy, thermotherapy ＋ ADM chemotherapy, and thermotherapy ＋ L-ADM chemotherapy on the cell proliferation and apoptosis were observed. The working concentration of ADM and L-ADM, and the cell proliferation rate were determined by MTT method. The apoptotic rate was determined by flow cytometry. Results The proliferation inhibition rate of thermotherapy ＋ L-ADM chemotherapy and thermotherapy ＋ ADM chemotherapy was 80.16% ± 3.78% and 62.09% ± 3.05%, respectively, and it was significantly higher than that of L-ADM chemotherapy （40.27% ± 2.32%）, ADM chemotherapy （30.56% ± 2.03%） or thermotherapy （16.51% ± 1.26%, P〈0.05）, and the proliferation inhibition rate of thermotherapy ＋ L-ADM chemotherapy was higher than that of thermotherapy ＋ ADM chemotherapy （P〈0.05）. The apoptotic rate of thermotherapy ＋ L-ADM chemotherapy and thermotherapy ＋ ADM chemotherapy was 84.19% ± 2.69% and 60.29% ±1.47%, respectively, and it was significantly higher than that of L-ADM chemotherapy （46.72% ± 2.09%）, ADM chemotherapy （35.09% ± 1.46%） and thermotherapy （17.85% ±0.78%, P〈0.05）, and the apoptotic rate ofthermotherapy ＋ L-ADM chemotherapy was higher than that of thermotherapy ＋ ADM chemotherapy （P〈0.05）. Conclusion Thermotherapy can enhance proliferation inhibition and apoptosis induction effect of ADM and L-ADM on SWO-38 glioma cells, and this effect is even stronger with L-ADM.