芦丁和维生素C的近红外漫反射光谱技术定量分析研究

Quantitative Analysis of Rutin and Vitamin C by NIR FTIR

首次利用近红外漫反射技术和偏最小二乘法定量分析了复方芦丁片的主组分芦丁和维生素C混合样品的含量 ,所建立的预测方程对样品的预测值和真实值之间的相关系数为 99 75 %,芦丁和维生素C定标标准差分别为 0 3 63 %和 1 0 78%。该方法快速、准确。

In this paper,near infrared diffuse reflectance spectroscopy(NIRDRS) method was used as a quantitative tool to measure the solid mixture of rutin and vitamin C by collecting NIR spectra in the range 7 000—3 900 cm -1 .The quantitative mathematics model was established with partial least squares(PLS) method.The correlation coefficient was 99 75%,the standard error of Rutin was 0 363%,and the standard error of Vitamin C was 1 078%.The methods are rapid,exact and nondestructive to samples.The components of the samples can be individually analyzed with high accuracy and precision,so NIRDRS can be a useful method of quality control of pharmaceuticals in the future.