纳米雄黄对肺癌A549细胞增殖影响及其机制的探讨

Effect of nanometer realgar Interfeing on A549 lung cancer cell proliferation and its mechanism

目的:探讨纳米雄黄对肺癌A549细胞增殖的影响及其可能机制。方法:制备纳米雄黄,体外培养肺癌A549细胞株。实验分组:对照组,纳米雄黄干预组(10%和25%纳米雄黄),DDP干预组(2μg/mL),10%联合组(10%纳米雄黄+DDP),25%联合组(25%纳米雄黄+DDP)。运用免疫组化法测定β-catenin蛋白表达情况,RT-PCR法检测c-mycmRNA表达。结果:肺癌A549细胞在加入纳米雄黄干预下,β-catenin蛋白表达与c-myc mRNA表达均受到不同程度的抑制,对照组、10%纳米雄黄组、25%纳米雄黄组、DDP组、10%联合组及25%联合组的β-catenin蛋白表达阳性率分别为(78.26±5.21)%、(53.64±5.57)%、(50.82±4.39)%、(45.26±3.75)%、(31.22±1.63)%和(24.07±1.15)%,c-mycmRNA相对表达量依次为(0.41±0.030)%、(0.35±0.015)%、(0.30±0.018)%、(0.26±0.019)%、(0.14±0.012)%及(0.06±0.010)%;与对照组相比,随着纳米雄黄药物浓度的增加及联用化疗药物,β-catenin蛋白表达阳性率逐渐降低,c-myc mRNA的表达量不断下降,纳米雄黄抑制作用与药物浓度呈依赖性,药物浓度越大抑制作用越明显,并且与化疗药DDP联用具有协同作用。结论:纳米雄黄可通过降低β-catenin及c-myc mRNA在肺癌A549细胞中的表达,阻断Wnt信号转导通路,有效抑制细胞增殖发挥抗肿瘤作用。

OBJECTIVE：To study the effect of nanometer realgar on A549 lung cancer cell proliferation and its possible mechanism. METHODS： Nanometer realgar was prepared, and A549 lung cancer cells was cultured in vitro. Experimental groups ： control group, 10 % and 25 % nanometer realgar intervention groups, 2μg/mE DDP group, 10 % joint group （10 % nanometer realgar ＋ DDP） and 25 % joint group（25 % nanometer realgar ＋ DDP）. Immunohistoehemical detection was used to analyze β-catenin protein expression, and RT-PCR detection was used to analyze c-myc mRNA expression. RESULTS： A549 lung cancer cells in add nanometer realgar intervention,β-catenin protein expression and c-mye mRNA expression were subject to different degrees of inhibition; A control group, 10% nm realgar group,25 % nm realgar group, DDP group, 10% joint group and 25% joint group of β-catenin positive rates were （78.26±5.21）%, （53.64±5.57）%, （50.82±4.39）%,（45.26±3.75） %, （31.22±1.63）%,（24.07± 1.15） % ; c-myc mRNA relative expression levels were followed by （0.41±0. 030） %,（0.35±0. 015） %,（0.30±0. 018）%, （0. 26±0. 019） %, （0.14±0. 012）% and （0.0±0. 010）%. Compared with the control, with the increasing of the concentration of nanometer realgar drugs and the application of combined chemotherapy,β-eatenin protein expression gradually reduce, c-myc mRNA expression quantity decreases, nanometer realgar inhibition and drug concentration in dependence, with the increase of drug concentration and inhibition enhance,and with chemotherapy drugs combined have a synergistic effect. CONCLUSION： Nanometer realgar can block Wnt signal transduction pathway by reduced β-catenin and c-myc mRNA in lung cancer A549 cells, can effectively inhibit the proliferation of anti tumor effect.