腺病毒介导的PML基因对银屑病样小鼠模型的作用

Effect of topical application of a recombinant adenovirus carrying promyelocytic leukemia gene in a psoriasis-like mouse model

目的研究腺病毒介导的PML基因对银屑病样小鼠模型的作用。方法采用小鼠阴道上皮及鼠尾鳞片表皮实验模型,观察PML基因重组腺病毒乳膏外用对鼠尾鳞片及阴道上皮细胞分裂的影响。结果 PML基因重组腺病毒乳膏具有显著抑制阴道上皮细胞有丝分裂、促进表皮颗粒层生成的作用。结论 PML基因对银屑病样小鼠模型的作用可能与促进表皮细胞正常分化和抑制表皮细胞增殖有关,PML基因局部外用有望用于银屑病等角化异常性疾病的治疗。

Objective To investigate the effects of topical treatment with adenovirus-mediated promyelocytic leukemia gene （PML） gene in a psoriasis-like mouse model. Methods The effect of adenovirus-mediated PML gene on the granular layer of mouse tail scale epidermis and epithelial mitosis were observed on longitudinal histological sections prepared from the tail skin and vaginal epithelium of the mice. Results Adenovirus-mediated PML gene significantly inhibited mitosis of mouse vaginal epithelial cells and promoted the formation of granular layer in mouse tail scale epidermis. Conclusion The therapeutic effect of PML gene in the psoriasis-like mouse model may be associated with increased granular cells and suppressed epidermic cell proliferation.