一株具有抗疟活性的金鸡纳树内生真菌的研究

Antimalarial substances of an endophytic fungus isolated from cinchona tree

为筛选具抗疟疾活性的的内生真菌,采用平板分离法对海南金鸡那树内生真菌进行分离纯化。利用"4日抑制疟疾"测试内生真菌发酵液的抗疟活性,采用TLC、HPLC等分析技术对内生菌发酵液等成分进行分析。从金鸡那树叶片分离到31种内生真菌,通过筛选找到了1株具有抗疟疾活性的产奎宁或奎宁类似物的内生真菌。从金鸡那树叶片中分离得到1株产奎宁的结实串孢霉属内生真菌,为奎宁的资源开发开辟了新途径。

Plate method was used to isolate and purify endophytic fungi from Cinchona sp. in Hainan Province. The anti-malaria activity of fermented liquid of the endophytic fungi was tested using “4 day inhibit malaria”, and the components such as quinine or structural analogues were analysed using TLC and HPLC. Of the 31 endophytic species isolated from cinchona tree one strain producing quinine or quinine analogues with antimalarial activity was obtained. Morphological observation indicated that this endophytic fungus could be identified as Hormiscium sp.