转录因子SOX4在云南宣威女性肺癌组织中的表达和临床意义

Expression of transcription factor SOX4 and its clinical significance in female lung cancer patients in Xuanwei area, Yunnan Province

目的探讨云南宣威女性肺癌组织中转录因子SOX4的表达与患者临床病理特征及预后的关系。方法应用实时荧光定量PCR和免疫组织化学技术检测96例宣威女性肺癌患者肿瘤组织和癌旁正常组织中SOX4mRNA和蛋白的表达水平，分析SOX4蛋白表达与患者临床病理特征及预后的关系。结果SOX4mRNA在宣威女性肺癌患者肿瘤组织和癌旁组织中的表达水平分别为2．53±1．65和1．43±1．14，差异有统计学意义（P=0．003）。SOX4蛋白在肿瘤组织和癌旁组织中的阳性表达率分别为53．1％（51／96）和26．0％（25／96），差异有统计学意义（P〈0．001）。SOX4蛋白的表达与肿瘤的临床分期、淋巴结转移以及分化程度有关（均P〈0．05）。单因素分析的结果显示，SOX4蛋白阳性表达、有淋巴结转移以及分期晚的患者中位生存时间较短（均P〈0．05）。Cox多因素分析的结果显示，临床分期为影响宣威女性肺癌患者预后的独立因素（P〈0．001）。结论SOX4mRNA和蛋白在宣威女性肺癌组织中的表达显著升高；SOX4蛋白阳性表达患者的中位生存时间较短；SOX4蛋白的表达水平结合临床分期可作为判断宣威女性肺癌患者预后的参考指标。

Objective To investigate the expression of transcription factor SOX4 in lung cancer tissues of female patients in Xuanwei area, Yunnan Province, and explore its correlation with clinicopathological characteristics and prognosis of the female patients. Methods Real-time PCR was applied on lung cancer specimens and their corresponding normal lung tissues from 96 female cases of Xuanwei area to assess the expression of SOX4 mRNA. Immunohistochemical staining was performed to investigate the SOX4 protein expression, and further to elucidate its correlation with clinicopathological characteristics and prognosis. Results The expression level of SOX4 mRNA in the cancer tissues （2.53 ±1.65 ） was significantly higher than that of matched normal tissues （ 1.43± 1. 14, P = 0. 003 ）. Immunohistochemical staining showed that there were 53.1% （51/96） positive expression of SOX4 protein in the cancer tissue and only 26.0% （25/96） in matched normal tissue （P 〈 0.001 ）. The expression of SOX4 protein had a significant correlation with clinical stage, lymph node metastasis and differentiation of tumor （ P 〈 0.05）. The survival analysis by Kaplan-Meier method showed that patients with positive expression of SOX4 protein, lymph node metastasis and advanced tumor stage had a significantly shorter median survival time （P 〈 0.05 ）. Cox regression survival analysis showed that pathological grade was a significant independent factor affecting prognosis. Conclusions The expressions of SOX4 mRNA and protein are significantly up-regulated in Xuanwei female lung cancer patients. Patients with positive SOX4 expression have a shorter median survival time. SOX4 protein expression level combined with pathological grade can be used as a prognostic indicator of female lung cancer patients in Xuanwei area, Yunnan Province.