用血清药理学方法观察芎芍胶囊对兔胸主动脉平滑肌细胞增殖凋亡的影响

Effect of Xiongshao Capsule on Proliferation and Apoptosis of Vascular Smooth Muscle Cells in Rabbits Observed by Serum Pharmacological Method

目的 :采用血清药理学方法观察芎芍胶囊对内皮素 (ET)致兔胸主动脉平滑肌细胞 (SMC)增殖凋亡的影响。方法 :分离正常兔胸主动脉SMC ,并传代培养 ,用ET造成胸主动脉SMC增殖模型。给正常兔灌胃芎芍胶囊混悬液 10天 ,制备含药血清 ,采用噻唑蓝 (MTT)比色法、流式细胞术及琼脂糖凝胶电泳法观察其对SMC增殖凋亡的影响。结果 :ET能明显促进SMC增殖 ,芎芍胶囊含药血清可减少S +G2 期SMC所占比例 ,对ET所致SMC增殖有明显抑制作用 ,并呈剂量依赖性 ,大剂量时尚可诱导SMC凋亡。结论 :芎芍胶囊可明显抑制ET所致SMC增殖 ,并有一定的诱导SMC凋亡的作用。

Objective: To observe the effect of Xiongshao Capsule (XSC) on proliferation and apoptosis of aortic smooth muscle cells (SMC) of rabbits by serum pharmacological method. Methods: SMC were isolated from thoracic aorta of the normal rabbits and passaged. The SMC proliferation model was established by endothelin (ET). The medicated serum was prepared after given the normal rabbits suspension of XSC through gastrogavage for 10 days. The proliferation and apoptosis of SMC were observed by methyl thiazolyl tetrazolium (MTT) colorimetry, flow cytometry and agarose gel electrophoresis. Results: ET obviously promoted proliferation of SMC, and the serum medicated with XSC could lower the proportion of SMC in S+G 2 phase and markedly in hibited the proliferation of SMC caused by ET in dose dependent manner. It also had certain effect on inducing apoptosis of SMC. Conclusion: XSC could markedly inhibit the proliferation of SMC caused by ET and induce apoptosis of SMC, which might be the mechanism of preventing coronary restenosis after interventional treatment.