龟鹿二仙胶及其拆方对去势骨关节炎大鼠关节软骨中Bax和Bcl-2的影响

Effects of Guilu Erxian Gelatin and its Disassembled Prescriptions on Bax and Bcl-2 of Articular Cartilage in Castrated Rat Models of Osteoarthritis

目的:通过观察龟鹿二仙胶方及其拆方对去势骨关节炎大鼠Bax与Bcl-2表达的影响,对比不同拆方抑制软骨细胞凋亡的作用。方法:选4月龄SD雌性大鼠造模,各药物干预2、4、8周后,免疫组化法观察关节软骨中Bax与Bcl-2的表达。结果:龟鹿二仙胶、龟甲加鹿角、龟鹿加人参、龟鹿加枸杞、盐酸氨基葡萄糖均可抑制模型大鼠软骨细胞Bax的表达和促进Bcl-2的表达,龟鹿二仙胶与龟鹿加枸杞效果相当,且优于龟甲加鹿角和龟鹿加人参。结论:配伍枸杞能显著提高龟甲和鹿角抑制软骨细胞凋亡的作用,是龟鹿二仙胶方中抑制凋亡的主配伍,而人参在这方面的作用不明显。

To observe the expression of Bax and Bcl-2 of articular cartilage in the castration osteoarthritis rats, which were treated by the Guilu Erxian glue and its disassembled prescriptions, and to compare the inhibition of chondro- cyte apoptosis among different prescriptions. Methods： Establish the castration model of osteoarthritis with four months fe- male SD rats. The expression of Bax and Bcl-2 of articular cartilage were detected by immunohistochemistry method 2, 4, and 8 weeks after treating with Guilu Erxian glue and its disassembled prescriptions respectively. Results： All of Guilu Erx- ian gelatin group, Guilu group, Guilushen group, Guiluqi group and GHC could inhibit the expression of Bax and promote the expression of Bcl-2 in model rats. Guilu Erxian gelatin group showed no difference with Guiluqi group, and both of them were better than Guilu group and Guilushen group. Conclusion：This study indicated that Gouqi combined with Guijia and Lujiao can inhibit effectively the apoptosis of chondrocyte. Guiluqi plays the first important role, but renshen is ineffec- tive.