小鼠富含半胱氨酸分泌蛋白-1DNA疫苗免疫避孕效果的评价

Immunocontracepive Effects of Mouse Cysteine-rich Secretory Protein-1 DNA Vaccine on Mice

目的:观察小鼠富含半胱氨酸分泌蛋白-1(Crisp-1)DNA避孕疫苗免疫雌、雄性BALB/c小鼠后特异性抗体的表达及免疫避孕效果。方法:将雌、雄性BALB/c小鼠随机分为实验组和对照组,每组雌鼠12只,雄鼠8只。每只动物经肌肉接种重组质粒pcDNA3.1-Crisp-1或pcDNA3.1空载体3次,ELISA测定小鼠血清中抗Crisp-1抗体水平的变化,Western blotting检测抗体的特异性。免疫结束后2周,将实验小鼠分别与未接种的正常雌、雄性小鼠合笼,记录每组雌鼠的妊娠率与每窝产仔数。结果:重组质粒pcDNA 3.1-Crisp-1可以在小鼠体内诱发特异性抗Crisp-1免疫应答,免疫后雌、雄性小鼠妊娠率和平均每窝产仔数均显著降低(P<0.05)。结论:重组质粒pcDNA3.1-Crisp-1具有一定的抗生育潜能。

Objective： To observe the expression of specific antibodies and immunological contracep- tive effects induced by cysteine-rich secretory protein-1 （Crisp-1） DNA vaccine on male and female BALB/c mice. Methods： pcDNA3.1-Crisp-1 group （8 males and 12 females） and Crisp-1 group （8 males and 12 females） received 3-time injections of pcDNA3.1-Crisp-1 or pcDNA3.1 vector, respectively. ELISA and Western blotting were used to examine the immune responses and immunoreactivity of antisera. Two weeks after the third immunizati0n~ all the mice were mated with the normal contra-sexual fertile mice and then the fertility rate and mean litter size were analyzed. Results： The recombinant plasmid pcDNA3.1-Crisp-1 can induce specific anti-Crisp-1 immune response in mice. pcDNA3.1-Crisp-1 injected animals presented a statistically significant reduction in their fertility Conclusion： The recombinant plasmid pcDNA3.1,Crisp-1 presented appreciable anti-fertility potentia＇i.＇