钙通道关闭及开放状态下稀土离子对淋巴细胞的跨膜行为

The Trans-Lymphocyte Membrane Behavior of Rare Earth When the Calcium Channel is Blocked and Opened

用荧光探针法(以Fura-2为荧光指示剂)通过监测细胞内荧光强度的变化来判断稀土的跨膜行为.在近生理条件下,从单细胞、多细胞两个角度,研究了人外周血淋巴细胞钙离子通道开放、关闭等不同状态Dy^(3+),La^(3+)等稀土离子的跨膜行为.实验得出:微量稀土离子在上述状态下均不能跨膜内流.我们还发现,在钙通道开放的情况下,低剂量稀土离子不影响细胞外钙跨膜内流.

We have investigated the transport of rare earth ions transporting across the membrane of human peripheral blood lymphocytes (HPBL) by monitoring the fluorescence of HPBL loaded with Fura - 2. Through unicellular and multicellular aspects we study the behaviors of transporting across the membrane of HPBL about La3 + , Dy3 + . In similar physiological conditions, when we activate the calcium channel of HPBL by mitogen: PHA and ConA, the lanthanum ions (0. 01mmol·L-1 , 0. 1mmol·L-1 , 1mmol·L-1 ) cannot pass the membrane through calcium ion entry; And when calcium entry blockers (CEBS) for example Verapamil and Nifedipine block the calcium channel, the lanthanum ions cannot enter across the membrane through other entries, either. It seems to us that the trace lanthanum cannot pass the membrane in above conditions. And we find the lanthanum cannot effect the extracelluar calcium influx across the plasma membrane through calcium entry.