摘要
目的:研究结肠癌转移相关基因1(MACC1)在胃癌组织中的表达及其临床意义。方法:采用RT-qPCR检测胃癌组织株和67例胃癌组织及癌旁正常组织中MACC1mRNA的表达,免疫组化技术检测67例胃癌组织及癌旁正常组织中MACC1蛋白的表达,并分析其与临床病理资料的关系。结果:BGC823、SGC7901和MGC803 3种胃癌细胞株中MACC1mRNA的相对表达量分别为3.458 4±0.047 9、4.768 5±0.100 6和4.257 5±0.029 5,差异有统计学意义,F=295.131,P<0.05。癌组织MACC1mRNA表达量(5.560 0±2.853 1)较癌旁正常组织(2.190 1±1.675 9)显著增高,t=6.907,P=0.000。肿瘤组织中MACC1蛋白的阳性率为64.18%(43/67),显著高于癌旁正常组织5.97%(4/67),χ2=49.844,P<0.05,且MACC1蛋白的异常高表达与分化程度(χ2=14.492,P=0.000)、腹膜转移(χ2=4.251,P=0.039)、淋巴转移(χ2=11.978,P=0.001)、临床分期(χ2=11.024,P=0.001)密切相关,而与年龄(χ2=0.129,P=0.720)、性别(χ2=0.407,P=0.523)、肿瘤大小(χ2=0.017,P=0.897)、部位(χ2=3.021,P=0.082)及远处转移(χ2=0.000,P=1.000)无关。结论:MACC1可以作为预测腹膜转移、淋巴转移,评价胃癌进展的有效指标,可为胃癌患者的临床诊疗提供依据。
OBJECTIVE: To investigate the expression and clinical implications of MACC1 in gastric cancer tissues. METHODS: The expression of MACC1 mRNA and protein in gastric cancer cell lines,gastric carcinoma and adjacent nor- mal tissues were detected by real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and immunohisto- chemistry method in 67 and 67 cases respectively. The relationship between MACC1 protein expression and the clinico- pathological features of patients with gastric cancer were analyzed. RESULTS:The expression levels of MACC1 mRNA in BGC823,SGC7901 and MGC803 were 3. 458 4±0. 047 9,4. 768 5±0.100 6 and 4. 257 5±0. 029 5,respectively. There were significant differences in the three gastric cancer cell lines(F=295. 131 ,P〈0. 05). In addition,the expression levels of MACC1 mRNA and protein were higher in gastric carcinoma(5. 560 0±2. 853 1) than those in adjacent normal tissues (2. 190 1±1. 675 9,t=6. 907,P=0. 000). The positive rates of MACC1 in gastric cancer tissues (64.18%0,43/67) were significantly higher than that in the adjacent normal tissues ( 5. 97 %, 4/67,χ2 = 49. 844, P 〈 0.05 ). The expression of MACC1 protein in gastric cancer tissue was evidently correlated with the degree of histological differentiation (χ2= 14. 492, P = 0. 000), the peritoneal metastasis( χ2 = 4.251, P = 0.039), the lymph node metastasis(χ2 = 11. 978, P = 0. 001 ) and the clinical stage(χ2 =11. 024,P=0. 001) ,but not with age (χ2 =0. 129,P=0. 720) ,gender (χ2 =0. 407,P=0. 523), tumor size (χ2 = 0. 017, P = 0. 897), location (χ2 = 3. 021, P = 0. 082) or distant metastasis ( χ2 = 0. 000, P = 1. 000). CONCLUSION: MACC1 as a predictor of peritoneal metastasis,lymph node metastasis and an effective indicator of the evaluation of gastric cancer progress,can provide the basis for clinical diagnosis and treatment of gastric cancer.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2013年第6期444-447,452,共5页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30960371)
甘肃卫生行业科研计划项目(GSWST-09-11)
关键词
胃肿瘤
结肠癌转移相关基因1
腹膜转移
淋巴转移
gastric neoplasms
metastasis associated with colon cancerl
peritoneal metastasis
lymph node metastasis