胃癌组织MACC1表达及其临床意义分析

Expression and clinical implications of MACC1 in gastric cancer tissues

目的:研究结肠癌转移相关基因1(MACC1)在胃癌组织中的表达及其临床意义。方法:采用RT-qPCR检测胃癌组织株和67例胃癌组织及癌旁正常组织中MACC1mRNA的表达,免疫组化技术检测67例胃癌组织及癌旁正常组织中MACC1蛋白的表达,并分析其与临床病理资料的关系。结果:BGC823、SGC7901和MGC803 3种胃癌细胞株中MACC1mRNA的相对表达量分别为3.458 4±0.047 9、4.768 5±0.100 6和4.257 5±0.029 5,差异有统计学意义,F=295.131,P<0.05。癌组织MACC1mRNA表达量(5.560 0±2.853 1)较癌旁正常组织(2.190 1±1.675 9)显著增高,t=6.907,P=0.000。肿瘤组织中MACC1蛋白的阳性率为64.18%(43/67),显著高于癌旁正常组织5.97%(4/67),χ2=49.844,P<0.05,且MACC1蛋白的异常高表达与分化程度(χ2=14.492,P=0.000)、腹膜转移(χ2=4.251,P=0.039)、淋巴转移(χ2=11.978,P=0.001)、临床分期(χ2=11.024,P=0.001)密切相关,而与年龄(χ2=0.129,P=0.720)、性别(χ2=0.407,P=0.523)、肿瘤大小(χ2=0.017,P=0.897)、部位(χ2=3.021,P=0.082)及远处转移(χ2=0.000,P=1.000)无关。结论:MACC1可以作为预测腹膜转移、淋巴转移,评价胃癌进展的有效指标,可为胃癌患者的临床诊疗提供依据。

OBJECTIVE： To investigate the expression and clinical implications of MACC1 in gastric cancer tissues. METHODS： The expression of MACC1 mRNA and protein in gastric cancer cell lines,gastric carcinoma and adjacent nor- mal tissues were detected by real-time fluorescence quantitative polymerase chain reaction （RT-qPCR） and immunohisto- chemistry method in 67 and 67 cases respectively. The relationship between MACC1 protein expression and the clinico- pathological features of patients with gastric cancer were analyzed. RESULTS：The expression levels of MACC1 mRNA in BGC823,SGC7901 and MGC803 were 3. 458 4±0. 047 9,4. 768 5±0.100 6 and 4. 257 5±0. 029 5,respectively. There were significant differences in the three gastric cancer cell lines（F=295. 131 ,P〈0. 05）. In addition,the expression levels of MACC1 mRNA and protein were higher in gastric carcinoma（5. 560 0±2. 853 1） than those in adjacent normal tissues （2. 190 1±1. 675 9,t=6. 907,P=0. 000）. The positive rates of MACC1 in gastric cancer tissues （64.18%0,43/67） were significantly higher than that in the adjacent normal tissues （ 5. 97 %, 4/67,χ2 = 49. 844, P 〈 0.05 ）. The expression of MACC1 protein in gastric cancer tissue was evidently correlated with the degree of histological differentiation （χ2= 14. 492, P = 0. 000）, the peritoneal metastasis（ χ2 = 4.251, P = 0.039）, the lymph node metastasis（χ2 = 11. 978, P = 0. 001 ） and the clinical stage（χ2 =11. 024,P=0. 001） ,but not with age （χ2 =0. 129,P=0. 720） ,gender （χ2 =0. 407,P=0. 523）, tumor size （χ2 = 0. 017, P = 0. 897）, location （χ2 = 3. 021, P = 0. 082） or distant metastasis （ χ2 = 0. 000, P = 1. 000）. CONCLUSION： MACC1 as a predictor of peritoneal metastasis,lymph node metastasis and an effective indicator of the evaluation of gastric cancer progress,can provide the basis for clinical diagnosis and treatment of gastric cancer.