中空纤维脂质体微筛选及其用于中药中香豆素类和木脂素类可透膜成分群的筛选和捕获

Hollow Fiber Liposome Microscreening and Its Application in Screening and Fishing Membrane Permeation Ingredient of Coumarins and Lignans in Traditional Chinese Medicine

建立了中空纤维脂质体微筛选(Hollow fiber liposome microscreening,HFLMS)研究中药透膜化合物的方法。以中空纤维为脂质体载体,在模拟人体自然吸收的条件下,对中药提取液中可透膜化合物进行了筛选,并用HPLC进行分析。本研究在对空白和脂质体中空纤维表面性质表征的基础上,对中空纤维活性中心与目标物的非特异性结合和HFLMS方法的重现性进行了考察;将HFLMS与HPLC结合,分别对中药材蛇床子、补骨脂、羌活、独活提取液中透过脂质体的香豆素类成分群和五味子提取液中透过脂质体的木脂素类成分群进行了筛选和捕获;通过与对照品的色谱保留时间比对,对筛选出的与脂质体结合的部分化合物进行了结构鉴别。

A novel method of hollow fiber liposome microscreening （HFLMS） coupled with HPLC was proposed and used in screening and analyzing membrane permeation ingredient group. In the HFLMS-HPLC, the hollow fiber filled with liposome was used as a screening device, the membrane permeation ingredient group were screened, fished and isolated from traditional Chinese medicines （TCMs） extracts in phosphate buffer （ pH 7.4）. The surface nature of hollow fiber with liposome was characterized ; the nonspecific binding between activity enter of hollow fiber and the target compound and the reproducibility of HFLMS-HPLC were studied. Using this method, the coumarins and lignans compound groups which could permeate through the liposome membrane were screened and fished from TCMs. By comparing the retention times with standards, several component structures of combined with liposomes were identified.