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RNAi在成体干细胞分化的研究进展

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摘要 RNAi(RNA interference)是普遍存在于自然界中的基因沉默现象,是近些年才发现并迅速发展起来的一门新兴的基因阻断技术,RNAi是一种强有效的抑制基因表达的工具,以高度特异性、高效性、作用迅速以及操作简单等特点受到分子生物学研究者们青睐,该技术由Andrew Fire和Craig Mello在秀丽新小杆线虫中发现,已广泛应用于基因功能的研究、抗肿瘤以及各种疾病的研究中。
出处 《吉林医学》 CAS 2013年第8期1520-1522,共3页 Jilin Medical Journal
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参考文献15

  • 1Mello CC,Conte DJ.Revealing the world of RNA interference[J].Nature,2004,431(7006):338.
  • 2Blau HM,Brazelton TR,Weimann JM.The evolving concept of astem cell:entity or function[J].Cell,2001,105(7):829.
  • 3Snippert HJ,Clevers H.Tracking adult stem cells[J].EMBORep,2011,12(2):113.
  • 4Hodgkinson T,Yuan XF,Bayat A.Adult stem cells in tissue.engineering[J].Expert Rev Med Devices,2009,6(6):621.
  • 5Low WC,Yau WW,Stanton LW,et al.Directing neuronaldifferentiation of primary neural progenitor cells by gene knockdownapproach[J].DNA Cell Biol,2012,31(7):1148.
  • 6李玉艳,梁志清,史常旭.造血干细胞基因转染研究进展[J].国际检验医学杂志,2009,30(4):355-357. 被引量:3
  • 7Gupta R,Hong D,Iborra F,et al.NOV(CCN3)functions as aregulator of human hematopoietic stem or progenitor cells[J].Science,2007,316(5824):590.
  • 8Papadaki M,Siapati EK,Vassilopoulos G.A foamy virus vectorsystem for stable and efficient RNAi expression in mammaliancells[J].Hum Gene Ther,2011,22(10):1293.
  • 9Martino S,Girolamo I,Tiribuzi R,et al.Efficient siRNA deliveryby the cationic liposome DOTAP in human hematopoieticstem cells differentiating into dendritic cells[J].J BiomedBiotechnol,2009,20(9):410.
  • 10Kim SU.Genetically engineered human neural stem cells forbrain repair in neurological diseases[J].Brain Dev,2007,29(4):193.

二级参考文献51

  • 1王丰,朱悦.RNA干扰沉默神经干细胞Nogo-66受体基因阻断Nogo-66抑制神经突生长作用的研究[J].中国修复重建外科杂志,2007,21(6):642-647. 被引量:5
  • 2Mei YF, Segerman A, Lindman K, et al. Human hematopoietic (CD34+) stem cells possess high affinity receptors for adenovirus type 11p[J].Virology,2004,328(2):198-207.
  • 3Wang H, Cao H, Wohlfahrt M, et al. Tightly regulated gene expression in human hematopoietic stem cells after transduction with helper dependent Ad5/35 vectors[J]. Exp Hematol,2008,36 (7) :823-831.
  • 4Lu ZZ,Ni F, Hu ZB,et al. Efficient gene transfer into hematopoietic cells by a retargeting adenoviral vector system with a chimeric fiber of adenovirus serotype 5 and 11p[J].Exp Hematol, 2006,34 (9):1170-1182.
  • 5Schroers R, Hildebrandt Y, Hasenkamp J, et al. Gene transfer into human T lymphocytes and natural killer cells by Ad5/F35 chimeric adenoviral vectors[J].Exp Hematol,2004,32(6):536-546.
  • 6Kelly PF, Radtke S, von Kalle C, et al. Stem cell collection and gene transfer in Fanconi anemia[J]. Mol Ther, 2007,15 ( 1 ) : 211- 219.
  • 7Hall KM, Horvath TL, Abonour R, et al. Decreased homing of retrovirally transduced human bone marrow CD34+ cells in the NOD/SCID mouse model[J]. Exp Hematol, 2006, 34 (4) : 433-442.
  • 8Crcareva A,Saito T,Kunisato A,et al. Hematopoietic stem cells expanded by fibroblast growth factor-1 are excellent targets for retrovirus-mediated gene delivery[J]. Exp Hematol, 2005, 33 (12) : 1459-1469.
  • 9Santoni de Sio F, Naldini L. Short-term culture of human CD34 + cells for lentiviral gene transfer[J].Methods Mol Biol, 2009,506 : 59-70.
  • 10Relander T,Johansson M,Olsson K,et al. Gene transfer to repop ulating human CD34+ cells using amphotropic, GALV -, or RD114-pseudotyped HIV1 based vectors from stable producer cells[J].Mol Ther,2005,11(3) :452 -459.

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