c-Met siRNA对U87-EGFRvⅢ胶质瘤细胞凋亡和增殖的影响

The effects of c-Met siRNA on apoptosis and proliferation of U87-EGFRvⅢ glioma cells

目的:合成针对c-Met的小干扰RNA(siRNA),研究其对U87-EGFRvⅢ(表皮生长因子受体Ⅲ型突变体)胶质瘤细胞凋亡和增殖的影响。方法:用脂质体转染合成的c-Met siRNA入U87-EGFRvⅢ细胞,未转染组为对照组,荧光实时定量PCR和Western blotting分别检测c-Met基因和蛋白的表达,MTT和Annexin V-FITC/PI法检测细胞增殖和凋亡情况。结果:c-Met siRNA明显抑制c-Met表达(P<0.05);明显增加细胞凋亡率(P<0.05)。同时,c-Met siRNA单独或协同EGFRvⅢsiRNA可抑制胶质瘤细胞增殖(P<0.05)。结论:c-Met siRNA可抑制U87-EGFRvⅢ胶质瘤细胞增殖、诱导其凋亡,为研究c-Met基因在胶质瘤中的作用及其靶向联合治疗提供了理论基础。

Objective To synthetize the small interfering RNA（siRNA） of c-Met and study its effects on apoptosis and proliteration of U87-EGFRv m glioma cells. Methods The synthesized e-Met siRNA was transfeeted into U87-EGFRvm cells by Lipofeetamine, non-transfection cells were control group. The expressions of c-Met gene and protein were tested by real time PCR and Western blotting.The cell proliferation and apoptosis were detected by. MTT and Annexin V-FITC/P1 assays respectively. Results c-Met siRNA significantly inhibited the expression of c-Met（P 〈 0.05）; c-Met siRNA significantly increased the cell apoptotie rates（P 〈 0.05）. Meanmile, c-Met siRNA alone or with EGFRvmsiRNA could inhibit the growth of glioma cells （P 〈 0.05）. Conclusion e-Met siRNA could inhibit the proliferation and induce apoptosis of U87-EGFRv m glioma cells, which provides a theoretical foundation for studying the effect of e-Met and combined target therapy in glioma.