经不同方式移植骨髓间充质干细胞治疗肝纤维化的疗效

Efficacy study of different transplanting ways of bone marrow mesenchymal stem cells in treatment of liver fibrosis

目的:探索经不同方式移植骨髓间充质干细胞(bone marrow stem cell,BMSC)治疗肝纤维化的疗效。方法:P4代骨髓间充质干细胞经培养并诱使其向肝细胞样细胞分化;通过CCL4制作大鼠肝纤维化模型。骨髓间充质干细胞通过静脉内、肝内、及腹腔注射等移植方式。通过组织学染色,免疫组织化学和生化分析比较不同移植方式对肝脏形态及功能的影响。结果:MSCs经诱导后不表达肝细胞标记物,而表达α-胎蛋白(AFP),白蛋白(ALB);组织学染色,免疫组化,及生化分析表明经静脉途径比其他途径更有利于挽救肝衰竭。结论:MSCs移植能抵抗肝脏纤维化,静脉移植方式是最有效的治疗途径。

Objective To explore the efficacy of different transplanting ways of bone marrow mesenchymal stem cells （BMSCs） in treatment of liver fibrosis. Methods The 4th generation BMSCs were cultured and induced into hepatocyte-fike cells. Liver fibrosis in SD rats was inducedby carbon tetrachloride. BMSCs were transplanted by intravenous injection, intrahepatic injection and intraperitoneal injection, etc. Histopathological staining, immunohistochemistry, and biochemical analysis were used to compare the liver morphological and Junctional innuenee among different transplanting ways. Results The induced MSCs did not express hepatoeyte markers, but which couldwhile expressed a-fetoprotein （AFP）, and albumin （ALB）. The intravenous injection way was more effective atbeneficial rescuing liver failure than other injection ways. Conclusions BMSCs transplantation was able to protect against liver fibrosis. Intravenous way was the most favorable transplanting approach.