阿尔茨海默病患者脑脊液miRNA检测及初步分析

MicroRNA profiling in cerebrospinal fluid in patients with Alzheimer′s disease and preliminary analysis

目的:分析AD患者特异性微小RNA(miRNA)表达谱,为深入研究miRNA与AD发生和发展的关系以及寻找新的AD分子标志物奠定基础。方法:利用miRNA芯片就10例AD患者和认知正常老年人脑脊液的miRNA表达谱进行检测和初步生物信息学分析,Real-TimePCR验证miRNA芯片检测结果。结果:miRNA芯片检测发现,在AD患者和认知正常老年人脑脊液中共有128个差异表达miRNA,其中63个上调,下调65个。另外,应用Real-TimePCR对miR-125b和miR-132这2个上调miRNA进行了验证,结果与芯片检测结果一致。结论:AD患者脑脊液具有特异性的miRNA表达谱,提示可能在miRNA表达环节找到新的AD分子标志物。

Objective To determine the microRNA（miRNA） expression profile of in CSF in AD patients. Methods miRNA microarrays were performed to detect the expression profile of miRNAs in 10 cases of CSF in AD patients and the healthy control persons. Preliminary bioinformatic analysis was conducted. The microarray results were confirmed by real-time PCR assay. Results miRNA microarray results revealed that 128 miRNAs were dysregulated in AD patients and the corresponding healthy controls. Sixty-three miRNAs were significantly up- regulated, and 65 miRNAs were significantly down-regulated. Real-time PCR results demonstrated that up- regulation of miR-125b and miR-132 was consistent with that based on microarray array. Conclusion Specific miRNA expression profile was determined in CSF in AD patients, and the differentially expressed miRNAs may be used as potential biomarkers of AD.