摘要
采用纤维蛋白铺盖和放射免疫方法研究了米非司酮 (RU486)对培养颗粒细胞及黄体细胞纤溶酶原激活因子 (包括组织型 t PA和尿激酶型 u PA)的作用。结果示 RU486能明显拮抗人绒毛膜促性腺激素 (h CG)促进颗粒细胞 t PA活性及孕酮分泌的作用。RU486和 PGF2α能明显促进黄体细胞 t PA分泌 ,但孕酮产生明显被抑制。用组织学培养的方法 ,结果表明 ,RU486能刺激妊娠子宫内膜 t PA和 u PA分泌。这些结果表明 RU486的抗生育作用部分能通过 PA起到抗排卵。
The effects of mifepristone (RU 486 ) on the productions of plasminogen activator and progesterone in rat granulosa cells and luteal cells were studied by fibrin overlay method and radioimmunoassay method. The results showed that RU 486 could significantly antagonize tPA activity and progesterone production enhanced by human chorionic gonadotropin (hCG). Secretion of tPA from luteal cells was significantly promoted by RU 486 with or without prostaglandin F 2α (PGF 2α ), but progesterone production in luteal cells was markedly inhibited with the same treatment. With method of tissue culture, the results showed that RU 486 was capable of stimulating tPA and urokinase (uPA) activity in endometrium of pregnant rats. These findings indicated that antifertility of RU 486 might be partly mediated through plasminogen activator plasminogen activator inhibitor system.
出处
《同济医科大学学报》
CSCD
2000年第5期402-404,共3页
Acta Universitatis Medicinae Tongji
