人肾癌组织EGFR和TGF-α的分布及其意义

The Distribution of EGFR and TGF α in Human Renal Carcinoma Tissues and Its Implication

对肾癌组织的表皮生长因子受体 (EGFR)和转化生长因子 α(TGF- α)的表达及 EGFR基因的转录进行研究 ,方法采用 L SAB免疫组化和 Digoxin标记原位杂交法 ,结果显示 :46例肾癌组织 EGFR和 TGF- α的表达阳性率明显高于 38例癌旁对照正常肾脏组织 ,EGFR为 5 4.3%和 2 1.0 % ,TGF- α为 39.1%和 13.2 % ,差异有显著性 (P<0 .0 5 ) ;部分癌组织 EGFR和 TGF- α同时表达 ;EGFR和 TGF- α的表达均与癌的分期、分级无关 ;石蜡包埋的 2 5例肾癌组织 EGFR- m RNA的原位杂交阳性率明显高于 2 0例癌旁正常肾组织 ,二者分别为 44 %和 15 % (P<0 .0 5 ) ,后者为弱阳性。认为肾癌组织可过度表达 EGFR和 TGF- α,并增强转录 EGFR- m RNA。过度表达的 EGFR或 TGF- α可能通过参与癌的自分泌生长环路而与癌的生长。

LSAB immunohistochemistry and digoxin labeled in situ hybridization methods were used to detect the expression of EGFR and TGF α and the transcription of EGFR mRNA in human renal cell carcinoma (RCC) tissues. The expression rate of EGFR and TGF α in 46 cases of human RCC tissues were significantly higher than that in 38 cases of corresponding autologous normal kidney tissues (EGFR: 53.4 % vs 21 0 %; TGF α: 39.1 % vs 13.2 %, P <0.05). Both EGFR and TGF α were simultaneously overexpressed in some cases of RCC tissues. No relationship existed between EGFR or TGF α and the RCC staging and grading. The positive rate of transcription EGFR mRNA in 25 cases of RCC tissues was significantly higher than that in 20 cases of corresponding autologous normal kidney tissues (44 % vs 15 %, P <0 05). The above findings demonstrated that RCC tissues overexpressed EGFR and TGF αand overtranscribed EGFR mRNA. The overexpressed EGFR and TGF α might contribute to the growth and development of RCC by taking part in the autocrine growth loop in RCC.