针叶小爪螨不同种群的酯酶同工酶比较

A COMPARATIVE STUDY ON ESTERASE ISOZYMES IN DIFFERENT POPULATIONS OF OLIGONYCHUS UNUNGUIS(JACOBI)

针叶小爪螨是我国南方用材树种杉木和北方经济林树种板栗上的重要害螨,为了解该螨不同种群间的遗传分化情况,采用垂直板型聚丙烯酰胺凝胶电泳技术对其雌成螨的酯酶同工酶进行了比较研究。结果表明:所有阔叶树种群的酶谱特征完全一致,未产生分化;2个针叶树种群(浙江杉木种群、吉林云杉种群)间以及针叶树种群与阔叶树间产生了明显的分化。

The spruce spider mite, Oligonychus ununguis （Jacobi） （Acari： Tetranychidae）, is the main pest mite of Chinese fir, Cunninghamia lanceolata, and chestnut, Castanea mollissima. Chinese fir is the timber tree species in southern China and chestnut is the economic tree species in northern China. Esterase isozymes in a- dult females from different populations of Oligonychus ununguis were studied by means of non - denaturing poly- acrylamide vertical slab gel electrophoresis for the purpose of understanding the genetic differentiations between different populations. Results showed that the eleetrophoretogram of esterase isozymes of all the deciduous popu- lations was same and no differentiation existed between different deciduous populations. There were significant differentiations between coniferous populations and deciduous populations. Significant differentiations were also found between two coniferous populations, fir population from Zhejiang Province and spruce population from Jilin Province.